Anti-Fibrillarin antibody (ab226178)
Key features and details
- Rabbit polyclonal to Fibrillarin
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Fibrillarin antibody
See all Fibrillarin primary antibodies -
Description
Rabbit polyclonal to Fibrillarin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, African green monkey, Orangutan, Giant Panda
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Immunogen
Synthetic peptide within Human Fibrillarin aa 271-321. The exact sequence is proprietary. (NP_001427.2).
Database link: P22087 -
Positive control
- WB: HeLa, HEK-293T, Jurkat and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226178 was affinity purified using an epitope specific to Fibrillarin immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Fibrillarin antibody (ab226178)All lanes : Anti-Fibrillarin antibody (ab226178) at 0.4 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 34 kDa
Exposure time: 10 seconds
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Immunoprecipitation - Anti-Fibrillarin antibody (ab226178)Fibrillarin was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226178 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226178 at 1 µg/ml.
Lane 1: ab226178 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
