Antibodies, Proteins, Kits and Reagents for Life Science

335 040 ₸ Product size

100 µl 335 040 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Fibrillarin antibody [EPR10822(B)] - Nucleolar Marker (ab154806)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10822(B)] to Fibrillarin - Nucleolar Marker
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Human

Product name

Anti-Fibrillarin antibody [EPR10822(B)] - Nucleolar Marker
See all Fibrillarin primary antibodies
Description

Rabbit monoclonal [EPR10822(B)] to Fibrillarin - Nucleolar Marker
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human Fibrillarin. The exact sequence is proprietary.
Positive control
- HeLa, HepG2, 293T and Molt4 cell lysates; Human colonic carcinoma and prostate tissues; HepG2 cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR10822(B)
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [EPR10822(B)] - Nucleolar Marker (ab154806)

Immunofluorescence analysis of HepG2 cells labeling Fibrillarin with ab154806 at 1/250 dilution.
Western blot - Anti-Fibrillarin antibody [EPR10822(B)] - Nucleolar Marker (ab154806)

All lanes : Anti-Fibrillarin antibody [EPR10822(B)] - Nucleolar Marker (ab154806) at 1/1000 dilution

Lane 1 : HeLa lysate
Lane 2 : HepG2 lysate
Lane 3 : 293T lysate
Lane 4 : Molt4 lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 33 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillarin antibody [EPR10822(B)] - Nucleolar Marker (ab154806)

Immunohistochemical analysis of paraffin-embedded Human colonic carcinoma tissue labeling Fibrillarin with ab154806 at 1/250 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillarin antibody [EPR10822(B)] - Nucleolar Marker (ab154806)

Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling Fibrillarin with ab154806 at 1/250 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillarin antibody [EPR10822(B)] - Nucleolar Marker (ab154806)

Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154806 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillarin antibody [EPR10822(B)] - Nucleolar Marker (ab154806)

Immunohistochemical analysis of paraffin embedded Human normal tonsil tissue using ab154806 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillarin antibody [EPR10822(B)] - Nucleolar Marker (ab154806)

Immunohistochemical analysis of paraffin embedded Human endometrial carcinoma tissue using ab154806 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillarin antibody [EPR10822(B)] - Nucleolar Marker (ab154806)

Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue using ab154806 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-Fibrillarin antibody [EPR10822(B)] - Nucleolar Marker (ab154806)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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