Anti-Fibrillarin antibody (ab137422)
Key features and details
- Rabbit polyclonal to Fibrillarin
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Fibrillarin antibody
See all Fibrillarin primary antibodies -
Description
Rabbit polyclonal to Fibrillarin -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 75-321 of Human Fibrillarin.
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Positive control
- 293T, H1299, MOLT4 and Neuro2A whole cell lysates; HeLa cells; NCIN87 Xenograft tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labelling Fibrillarin with ab137422 at 1/200 dilution. Lower image is merged with DNA probe.
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All lanes : Anti-Fibrillarin antibody (ab137422) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : H1299 whole cell lysate
Lane 3 : MOLT4 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 33 kDa
12% SDS PAGE -
Anti-Fibrillarin antibody (ab137422) at 1/1000 dilution + Neuro2A whole cell lysate at 30 µg
Predicted band size: 33 kDa
12 % SDS-PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillarin antibody (ab137422)Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft tissue labelling Fibrillarin with ab137422 at 1/100 dilution.