Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Fibrillarin antibody [4A4] (ab218846)

Key features and details

  • Mouse monoclonal [4A4] to Fibrillarin
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Fibrillarin antibody [4A4]
    See all Fibrillarin primary antibodies

  • Description

    Mouse monoclonal [4A4] to Fibrillarin

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant full length protein corresponding to Human Fibrillarin aa 1 to the C-terminus. (Purified from E.coli).
    Database link: P22087

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: NIH/3T3, HEK-293, HeLa and SH-SY5Y cell lysates. ICC/IF: HEK-293, C6 and HeLa cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [4A4] (ab218846)
    Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [4A4] (ab218846)

    C6 (rat glial tumor cell line) (Panel A) and HEK-293 (human epithelial cell line from embryonic kidney) (Panel B) cells stained for Fibrillarin (red) using ab218846 at 1/1000 dilution in ICC/IF. Co-stained with chicken pAb to vimentin, at 1/10000 dilution (green). The blue is DAPI staining of nuclear DNA.

  • Western blot - Anti-Fibrillarin antibody [4A4] (ab218846)
    Western blot - Anti-Fibrillarin antibody [4A4] (ab218846)
    All lanes : Anti-Fibrillarin antibody [4A4] (ab218846) at 1/2000 dilution

    Lane 1 : MW ladder
    Lane 2 : Rat whole brain lysate
    Lane 3 : Mouse whole brain lysate
    Lane 4 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
    Lane 5 : HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
    Lane 6 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 7 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) cell lysate

    Predicted band size: 33 kDa



    Fibrillarin detection is shown in green.

    Also stained with a rabbit antibody to UCHL-1 at 1/3000 dilution (red).

  • Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [4A4] (ab218846)
    Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [4A4] (ab218846)

    Immunofluorescent analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Fibrillarin with ab218846 at 1/1000 dilution (red). The cells were counterstained with a chicken antibody to vimentin at 1/5000 dilution (green) and DAPI to label the nuclei (blue).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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