Anti-Bcl-2 antibody [Bcl2/100] (ab117115)
![Anti-Bcl-2 antibody [Bcl2/100] (ab117115)](https://www.abcam.com/ps/products/117/ab117115/Images/ab117115-275404-anti-bcl-2-antibody-bcl2-100-western-blot.jpg "Anti-Bcl-2 antibody [Bcl2/100] (ab117115)")
Key features and details
- Mouse monoclonal [Bcl2/100] to Bcl-2
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Bcl-2 antibody [Bcl2/100]
See all Bcl-2 primary antibodies -
Description
Mouse monoclonal [Bcl2/100] to Bcl-2 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human Bcl-2 aa 1-100.
Database link: P10415
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab117115 is purified from cell culture supernatant by protein-A affinity chromatography. Purity is > 95% (by SDS-PAGE). -
Clonality
Monoclonal -
Clone number
Bcl2/100 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-Bcl-2 antibody [Bcl2/100] (ab117115)
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: BCL2 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: THP-1 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab117115 observed at 26 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab117115 was shown to specifically react with BCL2 when BCL2 knockout samples were used. Wild-type and BCL2 knockout samples were subjected to SDS-PAGE. Ab117115 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
