Recombinant human 4-1BBL protein (Fc Chimera Active) (ab220596)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE, ELISA
Preparation and Storage
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Images
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SDS-PAGE - Recombinant human 4-1BBL protein (Fc Chimera Active) (ab220596)
SDS-PAGE analysis of reduced ab220596 stained overnight with Coomassie Blue. The protein migrates as 52 kDa on a SDS-PAGE gel under reducing conditions.
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ELISA - Recombinant human 4-1BBL protein (Fc Chimera Active) (ab220596)Recombinant Human CD137 protein (His tag) (ab174028) at 0.1 μg/mL (100 μL/well) can bind ab220596 with a linear range of 0.2-6 ng/mL.
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Functional Studies - Recombinant human 4-1BBL protein (Fc Chimera Active) (ab220596)Loaded ab220596 on Protein A Biosensor, can bind Recombinant Human CD137 protein (His tag) (ab174028) with an affinity constant of 15.9 nM as determined in BLI assay.
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ELISA - Recombinant human 4-1BBL protein (Fc Chimera Active) (ab220596)Immobilized ab220596 at 2 μg/mL (100 μL/well) can bind Recombinant human CD137 protein (Active) (Biotin) (ab246104) with a linear range of 1-7.8 ng/mL.
