Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Fibrillarin antibody [EPR10823(B)] - BSA and Azide free (ab240147)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10823(B)] to Fibrillarin - BSA and Azide free
  • Suitable for: WB, Flow Cyt, ICC/IF, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Fibrillarin antibody [EPR10823(B)] - BSA and Azide free
    See all Fibrillarin primary antibodies

  • Description

    Rabbit monoclonal [EPR10823(B)] to Fibrillarin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab240147 is the carrier-free version of ab166630. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240147 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [EPR10823(B)] - BSA and Azide free (ab240147)
    Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [EPR10823(B)] - BSA and Azide free (ab240147)

    Immunocytochemistry/Immunofluorescence analysis of HT-29 (human colorectal adenocarcinoma) cells labelling Fibrillarin (green) with purified ab166630 at 1/5000. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as a nuclear counterstain.  

    Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab166630).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillarin antibody [EPR10823(B)] - BSA and Azide free (ab240147)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillarin antibody [EPR10823(B)] - BSA and Azide free (ab240147)

    ab166630 showing +ve staining in Human normal tonsil.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab166630).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillarin antibody [EPR10823(B)] - BSA and Azide free (ab240147)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillarin antibody [EPR10823(B)] - BSA and Azide free (ab240147)

    ab166630 showing +ve staining in Human melanoma.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab166630).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillarin antibody [EPR10823(B)] - BSA and Azide free (ab240147)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillarin antibody [EPR10823(B)] - BSA and Azide free (ab240147)

    ab166630 showing +ve staining in Human glioma.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab166630).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillarin antibody [EPR10823(B)] - BSA and Azide free (ab240147)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillarin antibody [EPR10823(B)] - BSA and Azide free (ab240147)

    ab166630 showing +ve staining in Human gastric adenocarcinoma.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab166630).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillarin antibody [EPR10823(B)] - BSA and Azide free (ab240147)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillarin antibody [EPR10823(B)] - BSA and Azide free (ab240147)

    Immunohistochemical analysis of paraffin embedded Human liver tissue labeling Fibrillarin with ab166630 at a 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab166630).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillarin antibody [EPR10823(B)] - BSA and Azide free (ab240147)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillarin antibody [EPR10823(B)] - BSA and Azide free (ab240147)

    Immunohistochemical analysis of paraffin embedded Human testis tissue labeling Fibrillarin with ab166630 at a 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab166630).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [EPR10823(B)] - BSA and Azide free (ab240147)
    Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [EPR10823(B)] - BSA and Azide free (ab240147)
    Immunofluorescent analysis of HeLa cells labeling Fibrillarin with ab166630 at a 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab166630).

  • Flow Cytometry - Anti-Fibrillarin antibody [EPR10823(B)] - BSA and Azide free (ab240147)
    Flow Cytometry - Anti-Fibrillarin antibody [EPR10823(B)] - BSA and Azide free (ab240147)
    Flow cytometric analysis of permeabilized MOLT4 cells using ab166630 at a 1/10 dilution (red) or a Rabbit IgG (negative) (green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab166630).

  • Anti-Fibrillarin antibody [EPR10823(B)] - BSA and Azide free (ab240147)
    Anti-Fibrillarin antibody [EPR10823(B)] - BSA and Azide free (ab240147)

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