Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Angiotensin Converting Enzyme 1 antibody [EPR22250-204] - BSA and Azide free (ab240561)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22250-204] to Angiotensin Converting Enzyme 1 - BSA and Azide free
Suitable for: WB, IHC-P, IHC-Fr, ELISA
Reacts with: Mouse, Rat

Product name

Anti-Angiotensin Converting Enzyme 1 antibody [EPR22250-204] - BSA and Azide free
See all Angiotensin Converting Enzyme 1 primary antibodies
Description

Rabbit monoclonal [EPR22250-204] to Angiotensin Converting Enzyme 1 - BSA and Azide free
Host species

Rabbit
Specificity

WB is only recommended for mouse.
Tested applications

Suitable for: WB, IHC-P, IHC-Fr, ELISAmore details
Species reactivity

Reacts with: Mouse, Rat
Immunogen

Recombinant fragment within Mouse Angiotensin Converting Enzyme 1 aa 600-1250. The exact sequence is proprietary.
Database link: P09470
Positive control
- IHC-P: Mouse lung tissue.
General notes
Ab240561 is the carrier-free version of ab222739. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab240561 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22250-204
Isotype

IgG
Research areas

ELISA - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22250-204] - BSA and Azide free (ab240561)

This data was developed using ab240561, the same antibody clone in a different buffer formulation.

ELISA analysis of Mouse ACE Recombinant Protein recombinant protein at 1000 ng/ml with ab222739. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22250-204] - BSA and Azide free (ab240561)

Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling Angiotensin Converting Enzyme 1 with ab222739 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on endothelial cells of rat lung (PMID: 19451697). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222739).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Frozen sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22250-204] - BSA and Azide free (ab240561)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney tissue labeling Angiotensin Converting Enzyme 1 with ab222739 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive staining on mouse renal tubules (PMID:25664248) is observed.

The nuclear counter stain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222739).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22250-204] - BSA and Azide free (ab240561)

Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Angiotensin Converting Enzyme 1 with ab222739 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on endothelial cells of mouse lung (PMID: 19451697). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240561).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-Angiotensin Converting Enzyme 1 antibody [EPR22250-204] - BSA and Azide free (ab240561)

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