Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414-152] - BSA and Azide free (ab250601)
![Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414-152] - BSA and Azide free (ab250601)](https://www.abcam.com/ps/products/250/ab250601/Images/ab250601-403046-anti-zonula-occludens-protein-3-zo3-antibody-epr13414-152-bsa-and-azide-free-western-blot.jpg "Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414-152] - BSA and Azide free (ab250601)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13414-152] to Zonula occludens protein 3/ZO3 - BSA and Azide free
- Suitable for: WB
- Reacts with: Rat, Human
Overview
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Product name
Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414-152] - BSA and Azide free
See all Zonula occludens protein 3/ZO3 primary antibodies -
Description
Rabbit monoclonal [EPR13414-152] to Zonula occludens protein 3/ZO3 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250601 is the carrier-free version of ab182159 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab250601 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as Zonula occludens protein 3
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR13414-152 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414-152] - BSA and Azide free (ab250601)All lanes : Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414-152] (ab182159) at 1/50000 dilution
Lane 1 : Caco-2 cell lysate
Lane 2 : MCF-7 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 103 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?This data was developed using ab182159, the same antibody clone in a different buffer formulation.
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![Western blot - Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414-152] - BSA and Azide free (ab250601)](https://www.abcam.com/ps/products/250/ab250601/Images/ab250601-398803-anti-zonula-occludens-protein-3-zo3-antibody-epr13414-152-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414-152] - BSA and Azide free (ab250601)Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414-152] (ab182159) at 1/10000 dilution + Human fetal kidney tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 103 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?This data was developed using ab182159, the same antibody clone in a different buffer formulation.
-
![Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414-152] - BSA and Azide free (ab250601)](https://www.abcam.com/ps/products/250/ab250601/Images/ab250601-3-benefits-of-recombinant-antibodies.png)
Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414-152] - BSA and Azide free (ab250601)