Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µl 328 339 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Angiotensin Converting Enzyme 1 antibody [EPR22250-204] (ab222739)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22250-204] to Angiotensin Converting Enzyme 1
Suitable for: WB, IHC-P, IHC-Fr, ELISA
Reacts with: Mouse, Rat, Recombinant fragment

Product name

Anti-Angiotensin Converting Enzyme 1 antibody [EPR22250-204]
See all Angiotensin Converting Enzyme 1 primary antibodies
Description

Rabbit monoclonal [EPR22250-204] to Angiotensin Converting Enzyme 1
Host species

Rabbit
Specificity

WB is only recommended for mouse.

Tested Applications & Species

Application	Species
ELISA	Recombinant fragment
IHC-Fr	Mouse
IHC-P	Mouse Rat
WB	Mouse

See all applications and species data

Immunogen

Recombinant fragment within Mouse Angiotensin Converting Enzyme 1 aa 600-1250. The exact sequence is proprietary.
Database link: P09470
Positive control
- WB: Mouse liver, brain, heart, kidney and lung lysates; bEnd.3 whole cell lysate. IHC-P: Mouse and rat lung tissues. IHC-Fr: Mouse kidney tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22250-204
Isotype

IgG
Research areas

ELISA - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22250-204] (ab222739)

ELISA analysis of Mouse ACE Recombinant Protein recombinant protein at 1000 ng/ml with ab222739. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
Western blot - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22250-204] (ab222739)

All lanes : Anti-Angiotensin Converting Enzyme 1 antibody [EPR22250-204] (ab222739) at 1/1000 dilution

Lane 1 : Mouse liver lysate
Lane 2 : Mouse brain lysate
Lane 3 : Mouse heart lysate
Lane 4 : Mouse kidney lysate
Lane 5 : bEnd.3 (mouse brain endothelioma cell line) whole cell lysate
Lane 6 : Mouse lung lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 151 kDa
Observed band size: 180 kDa
why is the actual band size different from the predicted?

Exposure time : Lane 1: 81 seconds; Lane 2: 26 seconds; Lane 3: 8 seconds; Lane 4: 3 seconds; Lane 5: 48 seconds; Lane 6: 10 seconds.

Blocking/Dilution buffer: 5% NFDM/TBST.

Apart from the target band at 180-kDa, weak reactivity bands observed could be due to glycosylation (PMID: 24163131).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22250-204] (ab222739)

Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Angiotensin Converting Enzyme 1 with ab222739 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on endothelial cells of mouse lung (PMID: 19451697). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Frozen sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22250-204] (ab222739)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney tissue labeling Angiotensin Converting Enzyme 1 with ab222739 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive staining on mouse renal tubules (PMID：25664248) is observed.

The nuclear counter stain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22250-204] (ab222739)

Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling Angiotensin Converting Enzyme 1 with ab222739 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on endothelial cells of rat lung (PMID: 19451697). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-Angiotensin Converting Enzyme 1 antibody [EPR22250-204] (ab222739)

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