Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Zyxin antibody [EPR4302] - BSA and Azide free (ab238430)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4302] to Zyxin - BSA and Azide free
  • Suitable for: WB, Flow Cyt, IHC-P, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Zyxin antibody [EPR4302] - BSA and Azide free
    See all Zyxin primary antibodies

  • Description

    Rabbit monoclonal [EPR4302] to Zyxin - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Mouse
    IP
    Mouse
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, MCF7 and C2C12 cell lysates; mouse lung and testis, rat lung and testis tissue lysates. IHC-P: Human gastric carcinoma, mouse and rat kidney tissue IP: Mouse testis tissue lysate. Flow Cyt: HeLa cells. ICC/IF: HeLa cells

  • General notes

    ab238430 is the carrier-free version of ab109316 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab238430 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-Zyxin antibody [EPR4302] - BSA and Azide free (ab238430)
    Western blot - Anti-Zyxin antibody [EPR4302] - BSA and Azide free (ab238430)
    All lanes : Anti-Zyxin antibody [EPR4302] (ab109316) at 1/20000 dilution (Purified)

    Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : C2C12 (Mouse myoblasts myoblast) whole cell lysates
    Lane 3 : Mouse lung lysates
    Lane 5 : Rat lung lysates
    Lane 6 : Rat testis lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 61 kDa
    Observed band size: 82 kDa
    why is the actual band size different from the predicted?



    We are unsure about the extra bands. They might be the cleavage fragments as what are described in PMID:17572661.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109316).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zyxin antibody [EPR4302] - BSA and Azide free (ab238430)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zyxin antibody [EPR4302] - BSA and Azide free (ab238430)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling Zyxin with Purified ab109316 at 1/1000 dilution (0.94 µg/mL). Heat mediated antigen retrieval using Bond™™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109316).

  • Immunocytochemistry/ Immunofluorescence - Anti-Zyxin antibody [EPR4302] - BSA and Azide free (ab238430)
    Immunocytochemistry/ Immunofluorescence - Anti-Zyxin antibody [EPR4302] - BSA and Azide free (ab238430)
    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Zyxin with Purified ab109316 at 1/500 dilution (1.87 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109316).
  • Western blot - Anti-Zyxin antibody [EPR4302] - BSA and Azide free (ab238430)
    Western blot - Anti-Zyxin antibody [EPR4302] - BSA and Azide free (ab238430)
    Anti-Zyxin antibody [EPR4302] (ab109316) at 1/20000 dilution (Purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 61 kDa
    Observed band size: 82 kDa why is the actual band size different from the predicted?



    We are unsure about the extra bands. They might be the cleavage fragments as what are described in PMID:17572661.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109316).

  • Immunoprecipitation - Anti-Zyxin antibody [EPR4302] - BSA and Azide free (ab238430)
    Immunoprecipitation - Anti-Zyxin antibody [EPR4302] - BSA and Azide free (ab238430)

    Purified ab109316 at 1/50 dilution (2ug) immunoprecipitating Zyxin in Mouse testis lysate.
    Lane 1 (input): Mouse testis lysate (10µg)
    Lane 2 (+): ab109316 + Mouse testis lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109316 in mouse testis lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/5000) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 82 kDa
    We are unsure about the extra bands. They might be the cleavage fragments as what are described in PMID:17572661
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109316).

  • Flow Cytometry - Anti-Zyxin antibody [EPR4302] - BSA and Azide free (ab238430)
    Flow Cytometry - Anti-Zyxin antibody [EPR4302] - BSA and Azide free (ab238430)
    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Zyxin with Purified ab109316 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109316).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zyxin antibody [EPR4302] - BSA and Azide free (ab238430)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zyxin antibody [EPR4302] - BSA and Azide free (ab238430)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling Zyxin with Purified ab109316 at 1/1000 dilution (0.94 µg/mL). Heat mediated antigen retrieval using Bond™™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109316).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zyxin antibody [EPR4302] - BSA and Azide free (ab238430)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zyxin antibody [EPR4302] - BSA and Azide free (ab238430)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gastric carcinoma tissue sections labeling Zyxin with Purified ab109316 at 1/1000 dilution (0.94 µg/mL). Heat mediated antigen retrieval using Bond™™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109316).

  • Anti-Zyxin antibody [EPR4302] - BSA and Azide free (ab238430)
    Anti-Zyxin antibody [EPR4302] - BSA and Azide free (ab238430)

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