Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Zyxin antibody [EPR4302] (ab109316)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4302] to Zyxin
Suitable for: WB, IP, IHC-P, Flow Cyt, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-Zyxin antibody [EPR4302]
See all Zyxin primary antibodies
Description

Rabbit monoclonal [EPR4302] to Zyxin
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Mouse Rat Human
IP	Mouse
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, MCF7 and C2C12 cell lysates; mouse lung and testis, rat lung and testis tissue lysates. IHC-P: Human gastric carcinoma, mouse and rat kidney tissue IP: Mouse testis tissue lysate. Flow Cyt: HeLa cells. ICC/IF: HeLa cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4302
Isotype

IgG
Research areas

Western blot - Anti-Zyxin antibody [EPR4302] (ab109316)

All lanes : Anti-Zyxin antibody [EPR4302] (ab109316) at 1/20000 dilution (Purified)

Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : C2C12 (Mouse myoblasts myoblast) whole cell lysates
Lane 3 : Mouse lung lysates
Lane 4 : Mouse testis lysates
Lane 5 : Rat lung lysates
Lane 6 : Rat testis lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 61 kDa
Observed band size: 82 kDa
why is the actual band size different from the predicted?

Blocking/Diluting buffer: 5% NFDM/TBST

We are unsure about the extra bands. They might be the cleavage fragments as what are described in PMID:17572661
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zyxin antibody [EPR4302] (ab109316)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gastric carcinoma tissue sections labeling Zyxin with purified ab109316 at 1/1000 dilution (0.94 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunocytochemistry/ Immunofluorescence - Anti-Zyxin antibody [EPR4302] (ab109316)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Zyxin with Purified ab109316 at 1/500 dilution (1.87 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunoprecipitation - Anti-Zyxin antibody [EPR4302] (ab109316)

Purified ab109316 at 1/50 dilution (2ug) immunoprecipitating Zyxin in Mouse testis lysate.
Lane 1 (input): Mouse testis lysate (10µg)
Lane 2 (+): ab109316 + Mouse testis lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109316 in mouse testis lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/5000) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 82 kDa
We are unsure about the extra bands. They might be the cleavage fragments as what are described in PMID:17572661
Flow Cytometry - Anti-Zyxin antibody [EPR4302] (ab109316)

Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Zyxin with Purified ab109316 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zyxin antibody [EPR4302] (ab109316)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling Zyxin with purified ab109316 at 1/1000 dilution (0.94 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zyxin antibody [EPR4302] (ab109316)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling Zyxin with purified ab109316 at 1/1000 dilution (0.94 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-Zyxin antibody [EPR4302] (ab109316)

Anti-Zyxin antibody [EPR4302] (ab109316) at 1/20000 dilution (Purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 61 kDa
Observed band size: 82 kDa why is the actual band size different from the predicted?

Blocking/Diluting buffer: 5% NFDM/TBST

We are unsure about the extra bands. They might be the cleavage fragments as what are described in PMID:17572661
Anti-Zyxin antibody [EPR4302] (ab109316)

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