Anti-Zyxin antibody - N-terminal (ab229757)
Key features and details
- Rabbit polyclonal to Zyxin - N-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Zyxin antibody - N-terminal
See all Zyxin primary antibodies -
Description
Rabbit polyclonal to Zyxin - N-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide within Human Zyxin (N terminal). The exact sequence is proprietary.
Database link: Q15942 -
Positive control
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Zyxin antibody - N-terminal (ab229757)All lanes : Anti-Zyxin antibody - N-terminal (ab229757) at 1/10000 dilution
Lane 1 : WT 293T cell extracts
Lane 2 : Zyxin Knockout 293T cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 61 kDa
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Western blot - Anti-Zyxin antibody - N-terminal (ab229757)All lanes : Anti-Zyxin antibody - N-terminal (ab229757) at 1/5000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 61 kDa7.5% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-Zyxin antibody - N-terminal (ab229757)4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Zyxin (green) using ab229757 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
