Anti-Zyxin (phospho S142) antibody (ab78910)
Key features and details
- Rabbit polyclonal to Zyxin (phospho S142)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Zyxin (phospho S142) antibody
See all Zyxin primary antibodies -
Description
Rabbit polyclonal to Zyxin (phospho S142) -
Host species
Rabbit -
Specificity
ab78910 detects endogenous levels of Zyxin only when phosphorylated at serine 142. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic phosphopeptide derived from human Zyxin around the phosphorylation site of serine 142 (K-V-SP-S-I)
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Positive control
- Extracts from Jurkat cells treated with paclitaxel (1uM, 24hours).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab78910 was purified by affinity chromatography using an epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Zyxin (phospho S142) antibody (ab78910)All lanes : Anti-Zyxin (phospho S142) antibody (ab78910) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells, treated
with paclitaxel (1uM, 24hours)
Lane 2 : Extracts from Jurkat cells, treated
with paclitaxel (1uM, 24hours) with immunising phosphopeptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 61 kDa
Observed band size: 61 kDa
