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Epigenetics and Nuclear Signaling Chromatin Binding Proteins DNA / RNA binding

Anti-CIRP antibody [EPR23628-36] (ab246510)

Price and availability

307 566 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-CIRP antibody [EPR23628-36] (ab246510)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23628-36] to CIRP
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat

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Overview

  • Product name

    Anti-CIRP antibody [EPR23628-36]
    See all CIRP primary antibodies
  • Description

    Rabbit monoclonal [EPR23628-36] to CIRP
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    IP
    Mouse
    WB
    Mouse
    Rat
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: NIH/3T3 and NIH/3T3 treated at 32°C for 48 hours, whole cell lysates; Mouse testis tissue lysate; Rat testis tissue lysate. IP: Mouse testis tissue lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR23628-36
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding
    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Other cell division antibodies

Images

  • Western blot - Anti-CIRP antibody [EPR23628-36] (ab246510)
    Western blot - Anti-CIRP antibody [EPR23628-36] (ab246510)
    All lanes : Anti-CIRP antibody [EPR23628-36] (ab246510) at 1/1000 dilution

    Lane 1 : Mouse testis tissue lysate
    Lane 2 : Rat testis tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 18 kDa
    Observed band size: 18 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.   

    Exposure time: 3 minutes.

  • Immunoprecipitation - Anti-CIRP antibody [EPR23628-36] (ab246510)
    Immunoprecipitation - Anti-CIRP antibody [EPR23628-36] (ab246510)

    CIRBP was immunoprecipitated from 0.35 mg Mouse testis tissue lysate with ab246510 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab246510 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse testis tissue lysate 10ug

    Lane 2: ab246510 IP in Mouse testis tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab246510 in Mouse testis tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 47 seconds.

    This blot was developed using a higher sensitivity ECL substrate.

  • Western blot - Anti-CIRP antibody [EPR23628-36] (ab246510)
    Western blot - Anti-CIRP antibody [EPR23628-36] (ab246510)
    All lanes : Anti-CIRP antibody [EPR23628-36] (ab246510) at 1/1000 dilution

    Lane 1 : NIH/3T3 (mouse embryonic fibroblast) treated at 37°C for 48 hours, whole cell lysate
    Lane 2 : NIH/3T3 treated at 32°C for 48 hours, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 18 kDa
    Observed band size: 18 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    This antibody also recognized an undetermined band at around 50 kDa 

    Exposure time: 3 minutes.

  • Anti-CIRP antibody [EPR23628-36] (ab246510)
    Anti-CIRP antibody [EPR23628-36] (ab246510)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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