Anti-CIRP antibody [EPR23628-36] (ab246510)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23628-36] to CIRP
- Suitable for: WB, IP
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-CIRP antibody [EPR23628-36]
See all CIRP primary antibodies -
Description
Rabbit monoclonal [EPR23628-36] to CIRP -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF MouseIP MouseWB MouseRat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: NIH/3T3 and NIH/3T3 treated at 32°C for 48 hours, whole cell lysates; Mouse testis tissue lysate; Rat testis tissue lysate. IP: Mouse testis tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23628-36 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-CIRP antibody [EPR23628-36] (ab246510) at 1/1000 dilution
Lane 1 : Mouse testis tissue lysate
Lane 2 : Rat testis tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
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CIRBP was immunoprecipitated from 0.35 mg Mouse testis tissue lysate with ab246510 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab246510 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse testis tissue lysate 10ug
Lane 2: ab246510 IP in Mouse testis tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab246510 in Mouse testis tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 47 seconds.
This blot was developed using a higher sensitivity ECL substrate.
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All lanes : Anti-CIRP antibody [EPR23628-36] (ab246510) at 1/1000 dilution
Lane 1 : NIH/3T3 (mouse embryonic fibroblast) treated at 37°C for 48 hours, whole cell lysate
Lane 2 : NIH/3T3 treated at 32°C for 48 hours, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a higher sensitivity ECL substrate.
This antibody also recognized an undetermined band at around 50 kDa
Exposure time: 3 minutes.
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