Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RBPMS2 antibody [EPR13121-79] (ab181098)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13121-79] to RBPMS2
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-RBPMS2 antibody [EPR13121-79]
    See all RBPMS2 primary antibodies

  • Description

    Rabbit monoclonal [EPR13121-79] to RBPMS2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal liver tissue, PC3 and HepG2 whole cell lysate (ab7900).

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13121-79

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-RBPMS2 antibody [EPR13121-79] (ab181098)
    Western blot - Anti-RBPMS2 antibody [EPR13121-79] (ab181098)
    All lanes : Anti-RBPMS2 antibody [EPR13121-79] (ab181098) at 2000 µg (purified)

    Lane 1 : PC-3 lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : Human fetal liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 22 kDa
    Additional bands at: 24 kDa (possible isoform), 26 kDa (possible isoform)



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-RBPMS2 antibody [EPR13121-79] (ab181098)
    Western blot - Anti-RBPMS2 antibody [EPR13121-79] (ab181098)
    Anti-RBPMS2 antibody [EPR13121-79] (ab181098) at 1/10000 dilution (purified) + Mouse heart tissue lysate at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 22 kDa
    Observed band size: 24 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-RBPMS2 antibody [EPR13121-79] (ab181098)
    Western blot - Anti-RBPMS2 antibody [EPR13121-79] (ab181098)
    Anti-RBPMS2 antibody [EPR13121-79] (ab181098) at 1/10000 dilution (purified) + Rat heart tissue lysate at 20 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 22 kDa
    Additional bands at: 24 kDa (possible isoform), 26 kDa (possible isoform)



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunoprecipitation - Anti-RBPMS2 antibody [EPR13121-79] (ab181098)
    Immunoprecipitation - Anti-RBPMS2 antibody [EPR13121-79] (ab181098)

    ab181098 (purified) at 1/50 immunoprecipitating RBPMS2 in HepG2 cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-RBPMS2 antibody [EPR13121-79] (ab181098)
    Western blot - Anti-RBPMS2 antibody [EPR13121-79] (ab181098)
    All lanes : Anti-RBPMS2 antibody [EPR13121-79] (ab181098) at 1/10000 dilution (unpurified)

    Lane 1 : PC3 cell lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 22 kDa

  • Western blot - Anti-RBPMS2 antibody [EPR13121-79] (ab181098)
    Western blot - Anti-RBPMS2 antibody [EPR13121-79] (ab181098)
    Anti-RBPMS2 antibody [EPR13121-79] (ab181098) at 1/10000 dilution (unpurified) + Human fetal liver lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 22 kDa

  • Anti-RBPMS2 antibody [EPR13121-79] (ab181098)
    Anti-RBPMS2 antibody [EPR13121-79] (ab181098)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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