Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸ 10 µl 127 315 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR2757] to Angiotensin Converting Enzyme 1
Suitable for: ELISA, WB, IHC-P
Reacts with: Human

Product name

Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757]
See all Angiotensin Converting Enzyme 1 primary antibodies
Description

Rabbit monoclonal [EPR2757] to Angiotensin Converting Enzyme 1
Host species

Rabbit
Tested Applications & Species

Application Species
ELISA
Human

IHC-P
Human

See all applications and species data
Immunogen

within Human Angiotensin Converting Enzyme 1 aa 1250 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P12821
Positive control
- Fetal kidney, fetal heart and fetal lung lysates; human kidney and spleen tissues.
General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
ELISA	Human
IHC-P	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR2757
Isotype

IgG
Research areas

ELISA - Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762)

ELISA analysis of Human ACE (angiotensin converting enzyme) (membrane form only) recombinant protein at 250ng/ml with ab75762. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
Western blot - Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762)

All lanes : Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762) at 1/1000 dilution

Lane 1 : fetal kidney lysate
Lane 2 : fetal heart lysate
Lane 3 : fetal lung lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 150 kDa
Observed band size: 195 kDa
why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762)

ab75762 at 1/100 dilution staining Angiotensin Converting Enzyme 1 in human kidney by Immunohistochemistry, Paraffin-embedded tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762)

ab75762 at 1/100 dilution staining Angiotensin Converting Enzyme 1 in human spleen by Immunohistochemistry, Paraffin-embedded tissue. Note positive staining of endothelial cells.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA - Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762)

ELISA analysis of Human ACE (angiotensin converting enzyme) (membrane form only) recombinant protein at 250 ng/mL with ab75762. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762)

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