Anti-PDGF B antibody (ab139753)
Key features and details
- Chicken Polyclonal to PDGF B
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgY
Overview
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Product name
Anti-PDGF B antibody
See all PDGF B primary antibodies -
Description
Chicken Polyclonal to PDGF B -
Host species
Chicken -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human PDGF B aa 100-200 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
(Peptide available asab140136) -
Positive control
- Ab139753 gave a positive signal when tested against PDGF BB recombinant proteins. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal kidney.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 3% BSA -
Concentration information loading... -
Purity
IgY fraction -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Images
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Western blot - Anti-PDGF BB antibody (ab139753)All lanes : Anti-PDGF B antibody (ab139753) at 1 µg/ml
Lane 1 : Recombinant Human PDGF B protein (ab73231)
Lane 2 :Recombinant human PDGF B protein (ab9706)
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 16.7 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutesab73231 has a His tag so shows a high MW.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGF BB antibody (ab139753)IHC image of PDGF BB staining in Human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab139753, 1µg/ml, for 15 mins at room temperature. A Goat anti-Chicken biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
