Anti-PDGF B antibody [EPR6834] (ab178409)
![Anti-PDGF B antibody [EPR6834] (ab178409)](https://www.abcam.com/ps/products/178/ab178409/Images/ab178409-383291-anti-pdgf-b-antibody-epr6834-westernblot-human-mouse-rat.jpg "Anti-PDGF B antibody [EPR6834] (ab178409)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6834] to PDGF B
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-PDGF B antibody [EPR6834]
See all PDGF B primary antibodies -
Description
Rabbit monoclonal [EPR6834] to PDGF B -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Synthetic peptide within Human PDGF B aa 200 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P01127 -
Positive control
- WB: Fetal brain tissue lysate, Human, Mouse and Rat brain tissue lysates, Mouse and Rat heart tissue lysates; A375 and HeLa cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6834 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PDGF B antibody [EPR6834] (ab178409)All lanes : Anti-PDGF B antibody [EPR6834] (ab178409) at 1/1000 dilution (Purified)
Lane 1 : Mouse heart lysates
Lane 2 : Rat heart lysates
Lane 3 : Human brain lysates
Lane 4 : Mouse brain lysates
Lane 5 : Rat brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 27 kDa
Observed band size: 27 kDaBlocking/Diluting Buffer and concentration: 5% NFDM/TBST
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![Western blot - Anti-PDGF B antibody [EPR6834] (ab178409)](https://www.abcam.com/ps/products/178/ab178409/Images/ab178409-206274-anti-pdgf-b-antibody-epr6834-western-blot.jpg)
Western blot - Anti-PDGF B antibody [EPR6834] (ab178409)All lanes : Anti-PDGF B antibody [EPR6834] (ab178409) at 1/1000 dilution (unpurified)
Lane 1 : Fetal brain lysate
Lane 2 : A375 lysate
Lane 3 : HeLa lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 27 kDa
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![Anti-PDGF B antibody [EPR6834] (ab178409)](https://www.abcam.com/ps/products/178/ab178409/Images/ab178409-6-benefits-of-recombinant-antibodies.png)
Anti-PDGF B antibody [EPR6834] (ab178409)
