Human VE Cadherin Matched Antibody Pair Kit (ab220121)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 500 pg/ml - 32000 pg/ml
Overview
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Product name
Human VE Cadherin Matched Antibody Pair Kit
See all VE Cadherin kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
52 pg/ml -
Range
500 pg/ml - 32000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human VE Cadherin Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human VE Cadherin.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab210968. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human VE Cadherin Capture Antibody 2 x 50µg 1 x 50µg Human VE Cadherin Detector Antibody 2 x 12.5µg 1 x 12.5µg Human VE Cadherin Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. -
Tissue specificity
Endothelial tissues and brain. -
Sequence similarities
Contains 5 cadherin domains. -
Post-translational
modificationsPhosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB. -
Cellular localization
Cell junction. Cell membrane. Found at cell-cell boundaries and probably at cell-matrix boundaries. - Information by UniProt
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Alternative names
- 7B 4
- 7B4
- 7B4 antigen
see all -
Database links
- Entrez Gene: 1003 Human
- Omim: 601120 Human
- SwissProt: P33151 Human
- Unigene: 76206 Human
Images
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Human VE Cadherin standard curve
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Human VE Cadherin Matched Antibody Pair Kit (ab220121)To learn more about the advantages of recombinant antibodies see here.
