Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] - BSA and Azide free (ab239883)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP913(2)Y] to E Cadherin (phospho S838 + S840) - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] - BSA and Azide free
    See all E Cadherin primary antibodies

  • Description

    Rabbit monoclonal [EP913(2)Y] to E Cadherin (phospho S838 + S840) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC: Human endometrium tissue, rat and mouse liver tissue; WB: Human, mouse, and rat brain tissue lysates.

  • General notes

    Ab239883 is the carrier-free version of ab76319. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab239883 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] - BSA and Azide free (ab239883)
    Western blot - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] - BSA and Azide free (ab239883)
    All lanes : Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319) at 1/500000 dilution (Purified)

    Lane 1 : Human brain lysate, membrane treated with Alkaline Phosphatase for 1 hour
    Lane 2 : Human brain lysate
    Lane 3 : Rat brain lysate, membrane treated with Alkaline Phosphatase for 1 hour
    Lane 4 : Rat brain lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 97 kDa
    Observed band size: 145 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab76319, the same antibody clone in a different buffer formulation.

    Blocking buffer: 5% NFDM/TBST

  • Immunohistochemistry paraffin embedded sections - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] - BSA and Azide free (ab239883)
    Immunohistochemistry paraffin embedded sections - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] - BSA and Azide free (ab239883)
    This data was developed using ab76319 the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling E Cadherin with Purified ab76319 at 1:80000 dilution (0.009 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] - BSA and Azide free (ab239883)
    Western blot - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] - BSA and Azide free (ab239883)
    Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319) at 1/10000 dilution (Purified) + Mouse brain lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 97 kDa
    Observed band size: 145 kDa why is the actual band size different from the predicted?



    This data was developed using ab76319, the same antibody clone in a different buffer formulation.

    Blocking buffer: 5% NFDM/TBST

  • Immunohistochemistry paraffin embedded sections - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] - BSA and Azide free (ab239883)
    Immunohistochemistry paraffin embedded sections - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] - BSA and Azide free (ab239883)
    This data was developed using ab76319 the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling E Cadherin with Purified ab76319 at 1:80000 dilution (0.009 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry paraffin embedded sections - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] - BSA and Azide free (ab239883)
    Immunohistochemistry paraffin embedded sections - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] - BSA and Azide free (ab239883)
    This data was developed using ab76319 the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium tissue sections labeling E Cadherin with Purified ab76319 at 1:80000 dilution (0.009 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] - BSA and Azide free (ab239883)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] - BSA and Azide free (ab239883) This image is courtesy of an anonymous Abreview.

    Unpurified ab76319 staining E Cadherin in mouse skin (pilosebaceous untis) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with Tween-20 and blocked with 10% normal donkey serum + 1% serum for 40 minutes at room temperature; antigen retrieval was by heat mediation in a citrate buffer, pH 6. Samples were incubated with primary antibody (1/400 in 1% BSA) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76319).

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