Anti-Neurofascin antibody [EPR19003] - BSA and Azide free (ab271948)
![Anti-Neurofascin antibody [EPR19003] - BSA and Azide free (ab271948)](https://www.abcam.com/ps/products/271/ab271948/Images/ab271948-3-ab184377266573antineurofascinantibodyepr19003immunohistochemistry.jpg "Anti-Neurofascin antibody [EPR19003] - BSA and Azide free (ab271948)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19003] to Neurofascin - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Neurofascin antibody [EPR19003] - BSA and Azide free
See all Neurofascin primary antibodies -
Description
Rabbit monoclonal [EPR19003] to Neurofascin - BSA and Azide free -
Host species
Rabbit -
Specificity
IHC is recommended for mouse and rat only.
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Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human brain and cerebellum lysates; Mouse and rat brain and spinal cord lysates. IHC-P: Mouse cerebrum and colon tissues. Rat cerebrum tissue.
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General notes
ab271948 is the carrier-free version of ab184377. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19003 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofascin antibody [EPR19003] - BSA and Azide free (ab271948)
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Neurofascin with ab184377 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasm staining on mouse cerebrum is observed [PMID: 23198679].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184377). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofascin antibody [EPR19003] - BSA and Azide free (ab271948)](https://www.abcam.com/ps/products/271/ab271948/Images/ab271948-2-ab184377266571antineurofascinantibodyepr19003immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofascin antibody [EPR19003] - BSA and Azide free (ab271948)Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling Neurofascin with ab184377 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasm and membrane staining on myenteric nerve plexus, and negative on epithelium cells and smooth muscle cells of mouse colon.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184377). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofascin antibody [EPR19003] - BSA and Azide free (ab271948)](https://www.abcam.com/ps/products/271/ab271948/Images/ab271948-1-ab184377266570antineurofascinantibodyepr19003immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofascin antibody [EPR19003] - BSA and Azide free (ab271948)Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Neurofascin with ab184377 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasm staining on rat cerebrum is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184377). -
![Anti-Neurofascin antibody [EPR19003] - BSA and Azide free (ab271948)](https://www.abcam.com/ps/products/271/ab271948/Images/ab271948-4-benefits-of-recombinant-antibodies.png)
Anti-Neurofascin antibody [EPR19003] - BSA and Azide free (ab271948)
