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Anti-Mago nashi homolog 2 antibody [EPR14037-43] (ab186431)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14037-43] to Mago nashi homolog 2
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Mago nashi homolog 2 antibody [EPR14037-43]
    See all Mago nashi homolog 2 primary antibodies

  • Description

    Rabbit monoclonal [EPR14037-43] to Mago nashi homolog 2

  • Host species

    Rabbit

  • Specificity

    The immunogen shares 100% homology with both MAGOHB (UniProt Q96A72) and MAGOH (UniProt P61326). We have not performed any tests in order to determine the cross-reactivity but it is possible that ab186431 could cross-react with both MAGOHB and MAGOH. Both proteins have a similar molecular weight of 17 kDa.

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, Jurkat, Raw264.7, PC12 and NIH/3T3 cell lysate. Human brain and Human prostatic hyperplasia tissue. HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Mago nashi homolog 2 antibody [EPR14037-43] (ab186431)
    Immunocytochemistry/ Immunofluorescence - Anti-Mago nashi homolog 2 antibody [EPR14037-43] (ab186431)

    Immunofluorescent analysis of 4% paraformaldehyde fixed HeLa cells labeling Mago nashi homolog 2 with ab186431 at 1/250 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution. Counterstained with Dapi blue.

  • Western blot - Anti-Mago nashi homolog 2 antibody [EPR14037-43] (ab186431)
    Western blot - Anti-Mago nashi homolog 2 antibody [EPR14037-43] (ab186431)
    All lanes : Anti-Mago nashi homolog 2 antibody [EPR14037-43] (ab186431) at 1/20000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 17 kDa
    Observed band size: 17 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mago nashi homolog 2 antibody [EPR14037-43] (ab186431)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mago nashi homolog 2 antibody [EPR14037-43] (ab186431)

    Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling Mago nashi homolog 2 with ab186431 at 1/1000 dilution. Counter stain Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mago nashi homolog 2 antibody [EPR14037-43] (ab186431)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mago nashi homolog 2 antibody [EPR14037-43] (ab186431)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Mago nashi homolog 2 with ab186431 at 1/1000 dilution. Counter stain Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-Mago nashi homolog 2 antibody [EPR14037-43] (ab186431)
    Western blot - Anti-Mago nashi homolog 2 antibody [EPR14037-43] (ab186431)
    All lanes :

    Lane 1 : Raw264.7 cell lysate
    Lane 2 : PC12 cell lysate
    Lane 3 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated

    Predicted band size: 17 kDa
    Observed band size: 17 kDa

  • Anti-Mago nashi homolog 2 antibody [EPR14037-43] (ab186431)
    Anti-Mago nashi homolog 2 antibody [EPR14037-43] (ab186431)

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