Anti-Neurofascin antibody [EPR19003] (ab184377)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19003] to Neurofascin
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Neurofascin antibody [EPR19003]
See all Neurofascin primary antibodies -
Description
Rabbit monoclonal [EPR19003] to Neurofascin -
Host species
Rabbit -
Specificity
IHC is recommended for mouse and rat only. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseRatWB MouseRatHuman -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human brain and cerebellum lysates; Mouse and rat brain and spinal cord lysates. IHC-P: Mouse cerebrum and colon tissues. Rat cerebrum tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19003 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Neurofascin antibody [EPR19003] (ab184377) at 1/2000 dilution
Lane 1 : Human brain lysate at 20 µg
Lane 2 : Human cerebellum lysate at 20 µg
Lane 3 : Human lung lysate at 20 µg
Lane 4 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 138 kDa
Observed band size: 125,155,186,75 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight and expression profile observed are consistent with the literatures (PMID: 23160985; PMID: 9562181).
The 75 kDa band represents one of the isoforms annotated in UniProt.
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Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Neurofascin with ab184377 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasm staining on mouse cerebrum is observed [PMID: 23198679].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Anti-Neurofascin antibody [EPR19003] (ab184377) at 1/2000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lane 3 : Mouse spinal cord lysate
Lane 4 : Rat spinal cord lysate
Lane 5 : Mouse lung lysate
Lane 6 : Mouse heart lysate
Lane 7 : Mouse kidney lysate
Lane 8 : Rat kidney lysate
Lane 9 : Rat spleen lysate
Lane 10 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 11 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 12 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 138 kDa
Observed band size: 125,155,186,75 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight and expression profile observed are consistent with the literatures (PMID: 23160985; PMID: 9562181).
The 75 kDa band represents one of the isoforms annotated in UniProt.
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Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling Neurofascin with ab184377 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasm and membrane staining on myenteric nerve plexus, and negative on epithelium cells and smooth muscle cells of mouse colon.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Neurofascin with ab184377 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasm staining on rat cerebrum is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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