Anti-Robo2 antibody (ab244331)
Key features and details
- Rabbit polyclonal to Robo2
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-Robo2 antibody
See all Robo2 primary antibodies -
Description
Rabbit polyclonal to Robo2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human Robo2 aa 900-1100.
Database link: Q9HCK4 -
Positive control
- WB: human cerebral cortex tissue; IHC-P: Mouse embryo E14, human cerebral cortex and placenta tissue; ICC: SH-SY5Y cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Robo2 antibody (ab244331)Anti-Robo2 antibody (ab244331) at 0.4 µg/ml + HEL (human bone marrow erythroleukemia cell line) whole cell lysate
Predicted band size: 151 kDa
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Robo2 antibody (ab244331)Immunohistochemical analysis of mouse embryo E14 tissue labeling Robo2 in developing olfatory bulb and olfactory nerve with ab244331 at 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunocytochemistry/ Immunofluorescence - Anti-Robo2 antibody (ab244331)Immunofluorescent analysis of SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells labeling Robo2 (green) in the nucleus with ab244331 at 2 µg/ml.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Robo2 antibody (ab244331)Immunohistochemical analysis of human cerebral cortex tissue labeling Robo2 in neuronal processes in neuropil with ab244331 at 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Robo2 antibody (ab244331)Immunohistochemical analysis of human placenta tissue labeling Robo2 in the cytoplasm of trophoblastic cels with ab244331 at 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Robo2 antibody (ab244331)Immunohistochemical analysis of human liver tissue labeling Robo2 (very weak positivity) with ab244331 at 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Robo2 antibody (ab244331)Immunohistochemical analysis of human kidney tissue labeling Robo2 with ab244331 at 1/20 dilution. Negative control.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
