Anti-Robo2 + Robo3 antibody (ab75014)
Key features and details
- Rabbit polyclonal to Robo2 + Robo3
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Robo2 + Robo3 antibody -
Description
Rabbit polyclonal to Robo2 + Robo3 -
Host species
Rabbit -
Specificity
Ab75014 has been shown to cross react with Robo2 and Robo3, but not Robo1. Robo3 expression is largely restricted to the developing cortex (PMID 30697732).
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Tested Applications & Species
See all applications and species dataApplication Species WB RatHuman
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Immunogen
Synthetic peptide corresponding to Mouse Robo2 + Robo3 aa 1250-1350 conjugated to keyhole limpet haemocyanin.
(Peptide available asab75013) -
Positive control
- This antibody gave a positive signal in the following tissue lysates: Rat Liver Mouse Liver
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75014 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB RatHumanApplication Abreviews Notes WB (2) Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 171 kDa (predicted molecular weight: 161 kDa).Notes WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 171 kDa (predicted molecular weight: 161 kDa).Target
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Relevance
The function of the nervous system depends on the precision of axon wiring during development. SLITs, a family of secreted chemorepellent proteins, are crucial for the proper development of several major forebrain tracts. They act as guidance cues in axonal migration/navigation during neural development, at the ventral midline of the neural tube. ROBO2 (Roundabout, axon guidance receptor, homolog 2) is a receptor for SLIT2, and probably SLIT1 and mediates the function of SLITs. ROBO3 is thought to be involved during neural development in axonal navigation at the ventral midline of the neural tube. In spinal chord development plays a role in guiding commissural axons probably by preventing premature sensitivity to Slit proteins thus inhibiting Slit signaling through ROBO1 (By similarity). Required for hindbrain axon midline crossing. -
Cellular localization
Membrane; Single-pass type I membrane protein. -
Database links
- Entrez Gene: 6092 Human
- Entrez Gene: 64221 Human
- Entrez Gene: 268902 Mouse
- Omim: 602431 Human
- SwissProt: Q96MS0 Human
- SwissProt: Q9HCK4 Human
- SwissProt: Q7TPD3 Mouse
- Unigene: 13305 Human
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Alternative names
- HGPPS 1 antibody
- HGPPS antibody
- HGPPS1 antibody
see all
Images
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Western blot - Anti-Robo2 + Robo3 antibody (ab75014)This image is courtesy of Alain Chédotal (Institut Vision, France)All lanes : Anti-Robo2 + Robo3 antibody (ab75014) at 1/500 dilution
Lane 1 : Un-transfected HEK293
Lane 2 : HEK293 cells expressing Robo 1
Lane 3 : HEK293 cells expressing Robo 2
Lane 4 : HEK293 cells expressing Robo 3
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 161 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking: 2.5% Milk block for 1 hour at 20°C
Secondary incubation: TBS +2.5% Milk block for 16 hours at 4°C
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Western blot - Anti-Robo2 + Robo3 antibody (ab75014)All lanes : Anti-Robo2 + Robo3 antibody (ab75014) at 1 µg/ml
Lane 1 : Liver (Rat) Tissue Lysate
Lane 2 : Liver (Mouse) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 161 kDa
Exposure time: 20 minutes
Datasheets and documents
References (4)
ab75014 has been referenced in 4 publications.
- Pinho AV et al. ROBO2 is a stroma suppressor gene in the pancreas and acts via TGF-ß signalling. Nat Commun 9:5083 (2018). PubMed: 30504844
- Xu R et al. Targeting skeletal endothelium to ameliorate bone loss. Nat Med 24:823-833 (2018). PubMed: 29785024
- Zeng Z et al. Slit2-Robo2 signaling modulates the fibrogenic activity and migration of hepatic stellate cells. Life Sci 203:39-47 (2018). PubMed: 29660433
- Rama N et al. Slit2 signaling through Robo1 and Robo2 is required for retinal neovascularization. Nat Med 21:483-91 (2015). PubMed: 25894826
Images
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Western blot - Anti-Robo2 + Robo3 antibody (ab75014) This image is courtesy of Alain Chédotal (Institut Vision, France)All lanes : Anti-Robo2 + Robo3 antibody (ab75014) at 1/500 dilution
Lane 1 : Un-transfected HEK293
Lane 2 : HEK293 cells expressing Robo 1
Lane 3 : HEK293 cells expressing Robo 2
Lane 4 : HEK293 cells expressing Robo 3
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 161 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking: 2.5% Milk block for 1 hour at 20°C
Secondary incubation: TBS +2.5% Milk block for 16 hours at 4°C
-
Western blot - Anti-Robo2 + Robo3 antibody (ab75014)All lanes : Anti-Robo2 + Robo3 antibody (ab75014) at 1 µg/ml
Lane 1 : Liver (Rat) Tissue Lysate
Lane 2 : Liver (Mouse) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 161 kDa
Exposure time: 20 minutes
