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Signal Transduction Protein Phosphorylation Tyrosine Kinases Receptor Tyrosine Kinases

Recombinant human EGFR protein (Mutant) (ab208473)

Recombinant human EGFR protein (Mutant) (ab208473)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: > 75% Densitometry
  • Active: Yes
  • Tags: GST tag N-Terminus
  • Suitable for: SDS-PAGE, Functional Studies

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Description

  • Product name

    Recombinant human EGFR protein (Mutant)
    See all EGFR proteins and peptides
  • Biological activity

    The specific activity of ab208473 was determined to be 90 nmol/min/mg.

  • Purity

    > 75 % Densitometry.

  • Expression system

    Baculovirus infected Sf9 cells
  • Accession

    P00533
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAI KEATSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGC LLDYVREHKDNIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKT PQHVKITDFGLAKLLGAEEKEYHAEGGKVPIKWMALESILHRIYTHQSDV WSYGVTVWELMTFGSKPYDGIPASEISSILEKGERLPQPPICTIDVYMIM VKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHLPSPTDSNF YRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTV ACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSV PKRPAGSVQNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCV NSTFDSPAHWAQKGSHQISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEY LRVAPQSSEFIGA
    • Predicted molecular weight

      87 kDa including tags
    • Amino acids

      695 to 1210
    • Tags

      GST tag N-Terminus
    • Additional sequence information

      Mutation (d747-749) (NM_005228).

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 7.50
    Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.002% PMSF, 0.004% DTT, 25% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

Images

  • SDS-PAGE - Recombinant human EGFR protein (Mutant) (ab208473)
    SDS-PAGE - Recombinant human EGFR protein (Mutant) (ab208473)

    SDS-PAGE analysis of ab208473.

  • Functional Studies - Recombinant human EGFR protein (Mutant) (ab208473)
    Functional Studies - Recombinant human EGFR protein (Mutant) (ab208473)

    Sample Kinase Activity assay plot using ab208473, showing the specific activity to be 90 nmol/min/mg.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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