Anti-RANK antibody [EPR4740(N)] - BSA and Azide free (ab250600)
![Anti-RANK antibody [EPR4740(N)] - BSA and Azide free (ab250600)](https://www.abcam.com/ps/products/250/ab250600/Images/ab250600-403041-anti-rank-antibody-epr4740-n-bsa-and-azide-free-western-blot.jpg "Anti-RANK antibody [EPR4740(N)] - BSA and Azide free (ab250600)"){kind=icon}
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4740(N)] to RANK - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-RANK antibody [EPR4740(N)] - BSA and Azide free
See all RANK primary antibodies -
Description
Rabbit monoclonal [EPR4740(N)] to RANK - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250600 is the carrier-free version of ab182158. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250600 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR4740(N) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RANK antibody [EPR4740(N)] - BSA and Azide free (ab250600)All lanes : Anti-RANK antibody [EPR4740(N)] (ab182158) at 1/10000 dilution
Lane 1 : Human adrenal gland tissue lysate
Lane 2 : Human fetal liver tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size: 66 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?This data was developed using ab182158, the same antibody clone in a different buffer formulation.
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![Western blot - Anti-RANK antibody [EPR4740(N)] - BSA and Azide free (ab250600)](https://www.abcam.com/ps/products/250/ab250600/Images/ab250600-398801-anti-rank-antibody-epr4740-n-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-RANK antibody [EPR4740(N)] - BSA and Azide free (ab250600)All lanes : Anti-RANK antibody [EPR4740(N)] (ab182158) at 1/2000 dilution
Lane 1 : Human small intestine tissue lysate
Lane 2 : Human fetal thymus tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size: 66 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?This data was developed using ab182158, the same antibody clone in a different buffer formulation.
-
![Anti-RANK antibody [EPR4740(N)] - BSA and Azide free (ab250600)](https://www.abcam.com/ps/products/250/ab250600/Images/ab250600-3-benefits-of-recombinant-antibodies.png)
Anti-RANK antibody [EPR4740(N)] - BSA and Azide free (ab250600)
