Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5873(2)] to 14-3-3 sigma/SFN
  • Suitable for: WB, IHC-P
  • Reacts with: Human, Recombinant fragment

Overview

  • Product name

    Anti-14-3-3 sigma/SFN antibody [EPR5873(2)]
    See all 14-3-3 sigma/SFN primary antibodies

  • Description

    Rabbit monoclonal [EPR5873(2)] to 14-3-3 sigma/SFN

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human skin, A431 and HACAT lysates; Human 14-3-3 Sigma/SFN recombinant protein; Human tonsil tissue.

  • General notes

     

     

     This product was previously labelled as 14-3-3 sigma

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667)
    Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667)
    All lanes : Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667) at 1/1000 dilution

    Lane 1 : Human skin lysate
    Lane 2 : A431 cell lysate
    Lane 3 : HACAT cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 27 kDa
    Observed band size: 28 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling 14-3-3 sigma/SFN with ab193667 at 1/600 dilution (2μg/ml), followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667)
    Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667)
    Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667) at 1/1000 dilution + Human 14-3-3 sigma/SFN recombinant protein with His tag at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 27 kDa
    Observed band size: 28 kDa why is the actual band size different from the predicted?



    Human full length 14-3-3 sigma/SFN recombinant protein is a house-made one with His-Tag.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667)
    Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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