Anti-14-3-3 sigma/SFN antibody (ab77187)
Key features and details
- Goat polyclonal to 14-3-3 sigma/SFN
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-14-3-3 sigma/SFN antibody
See all 14-3-3 sigma/SFN primary antibodies -
Description
Goat polyclonal to 14-3-3 sigma/SFN -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Sheep, Cow, Dog, Pig, Zebrafish, Dictyostelium discoideum, Rainbow trout
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Immunogen
Synthetic peptide corresponding to Human 14-3-3 sigma/SFN (internal sequence).
Sequence:DLHTLSEDSYKDST
Database link: P31947 -
General notes
This product was previously labelled as 14-3-3 sigma
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-14-3-3 sigma/SFN antibody (ab77187)Anti-14-3-3 sigma/SFN antibody (ab77187) at 0.03 µg/ml + Human skin tissue lysate in RIPA buffer at 35 µg
Predicted band size: 28 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour. Detected using chemiluminescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody (ab77187)Immunohistochemical analysis of paraffin embedded Human Skin tissue labeling 14-3-3 sigma/SFN with ab77187 at 2 ug/ml. Tissue underwent antigen retrieval in steam with Tris/EDTA buffer (pH 9.0). The HRP-staining procedure was used for detection.
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Western blot - Anti-14-3-3 sigma/SFN antibody (ab77187)All lanes : Anti-14-3-3 sigma/SFN antibody (ab77187) at 0.03 µg/ml
Lane 1 : A431 cell lysate in RIPA buffer
Lane 2 : U2OS cell lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 28 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour. Detected using chemiluminescence.
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Western blot - Anti-14-3-3 sigma/SFN antibody (ab77187)All lanes : Anti-14-3-3 sigma/SFN antibody (ab77187) at 0.03 µg/ml
Lane 1 : Mouse skin tissue lysate in RIPA buffer
Lane 2 : Rat skin tissue lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 28 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour. Detected using chemiluminescence.
