Anti-ErbB3 / HER3 (phospho Y1328) antibody (ab131444)
Key features and details
- Rabbit polyclonal to ErbB3 / HER3 (phospho Y1328)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ErbB3 / HER3 (phospho Y1328) antibody
See all ErbB3 / HER3 primary antibodies -
Description
Rabbit polyclonal to ErbB3 / HER3 (phospho Y1328) -
Host species
Rabbit -
Specificity
ab131444 detects endogenous levels of ErbB3 / HER3 only when phosphorylated at Tyrosine 1328.
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Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human ErbB3/ HER3 conjugated to keyhole limpet haemocyanin. Synthetic phosphopeptide conjugated to KLH, surrounding the phosphorylation site of Tyrosine 1328 (
P-D-Y(p)-W-H
) of Human ErbB3/ HER3.
Sequence:P-D-Y(p)-W-H
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Positive control
- A431 cell extract treated with EGF; MCF7 cells; Human breast carcinoma tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol
PBS is without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab131444 was purified by using epitope specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-ErbB3 / HER3 (phospho Y1328) antibody (ab131444) at 1/500 dilution
Lane 1 : A431 cell extract, untreated
Lane 2 : A431 cell extract, treated with EGF
Predicted band size: 148 kDa
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Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling ErbB3 / HER3 (phospho Y1328) with ab131444 antibody at 1/50 dilution. The image on the right is treated with the synthesized peptide.
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Immunofluorescence of methanol-fixed MCF7 cells labelling EErbB3 / HER3 (phospho Y1328) with ab131444 antibody at 1/100 dilution.