Anti-14-3-3 sigma/SFN antibody (ab94490)
Key features and details
- Rabbit polyclonal to 14-3-3 sigma/SFN
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-14-3-3 sigma/SFN antibody
See all 14-3-3 sigma/SFN primary antibodies -
Description
Rabbit polyclonal to 14-3-3 sigma/SFN -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human 14-3-3 sigma/SFN aa 150 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab105198) -
Positive control
- This antibody gave a positive signal in Human skin tissue lysate.
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General notes
This product was previously labelled as 14-3-3 sigma
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab94490 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatSheepCowPigApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).Target
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Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.
p53-regulated inhibitor of G2/M progression. -
Tissue specificity
Present mainly in tissues enriched in stratified squamous keratinizing epithelium. -
Sequence similarities
Belongs to the 14-3-3 family. -
Post-translational
modificationsUbiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation. -
Cellular localization
Cytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway. - Information by UniProt
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Database links
- Entrez Gene: 528453 Cow
- Entrez Gene: 2810 Human
- Entrez Gene: 55948 Mouse
- Entrez Gene: 733625 Pig
- Entrez Gene: 313017 Rat
- Entrez Gene: 443032 Sheep
- Omim: 601290 Human
- SwissProt: Q0VC36 Cow
see all -
Alternative names
- 14 3 3 protein sigma antibody
- 14-3-3 protein sigma antibody
- 1433S_HUMAN antibody
see all
Images
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Western blot - Anti-14-3-3 sigma/SFN antibody (ab94490)Anti-14-3-3 sigma/SFN antibody (ab94490) at 1 µg/ml + Human skin tissue lysate - total protein (ab30166) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 28 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
Western blot - Anti-14-3-3 sigma/SFN antibody (ab94490)
Datasheets and documents
References (0)
ab94490 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-14-3-3 sigma/SFN antibody (ab94490)Anti-14-3-3 sigma/SFN antibody (ab94490) at 1 µg/ml + Human skin tissue lysate - total protein (ab30166) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 28 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
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Western blot - Anti-14-3-3 sigma/SFN antibody (ab94490)
