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Neuroscience Neurology process Neural Signal Transduction

Anti-14-3-3 sigma/SFN antibody (ab151504)

Anti-14-3-3 sigma/SFN antibody (ab151504)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to 14-3-3 sigma/SFN
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-14-3-3 sigma/SFN antibody
    See all 14-3-3 sigma/SFN primary antibodies
  • Description

    Rabbit polyclonal to 14-3-3 sigma/SFN
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human 14-3-3 sigma/SFN aa 154-247.
    Database link: P31947

  • Positive control

    • 293T, A431, HeLa, HepG2, Neuro2A, GL261 and PC12 whole cell lysates; A431 cells; Human 59T xenograft tissue.
  • General notes

     This product was previously labelled as 14-3-3 sigma

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neural Signal Transduction
    • Signal Transduction
    • Adapters
    • Cytoplasmic
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • 14-3-3

Images

  • Western blot - Anti-14-3-3 sigma/SFN antibody (ab151504)
    Western blot - Anti-14-3-3 sigma/SFN antibody (ab151504)
    All lanes : Anti-14-3-3 sigma/SFN antibody (ab151504) at 1/3000 dilution

    Lane 1 : Neuro2A whole cell lysate
    Lane 2 : GL261 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 27 kDa



    12% SDS PAGE
  • Western blot - Anti-14-3-3 sigma/SFN antibody (ab151504)
    Western blot - Anti-14-3-3 sigma/SFN antibody (ab151504)
    Anti-14-3-3 sigma/SFN antibody (ab151504) at 1/3000 dilution + PC12 whole cell lysate at 30 µg

    Predicted band size: 27 kDa



    12% SDS PAGE
  • Western blot - Anti-14-3-3 sigma/SFN antibody (ab151504)
    Western blot - Anti-14-3-3 sigma/SFN antibody (ab151504)
    All lanes : Anti-14-3-3 sigma/SFN antibody (ab151504) at 1/3000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 27 kDa



    12% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 sigma/SFN antibody (ab151504)
    Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 sigma/SFN antibody (ab151504)

    Immunofluorescent analysis of A431 cells (fixed in ice-cold Methanol for 5 min), labeling 14-3-3 sigma/SFN with ab151504 at 1/500 dilution. The right image shows cells co-stained with Hoechst 33343.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody (ab151504)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody (ab151504)

    Immunohistochemical analysis of paraffin-embedded Human 59T xenograft tissue, labeling 14-3-3 sigma/SFN with ab151504 at 1/100 dilution.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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