Anti-14-3-3 sigma/SFN antibody (ab86380)
Key features and details
- Rabbit polyclonal to 14-3-3 sigma/SFN
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-14-3-3 sigma/SFN antibody
See all 14-3-3 sigma/SFN primary antibodies -
Description
Rabbit polyclonal to 14-3-3 sigma/SFN -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to Human 14-3-3 sigma/SFN aa 198-248.
Database link: NP_006133.1 -
Positive control
- HeLa whole cell lysate (ab150035)
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General notes
This product was previously labelled as 14-3-3 sigma
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-14-3-3 sigma/SFN antibody (ab86380) at 0.1 µg/ml
Lane 1 : Hela whole cell lysate at 50 µg
Lane 2 : Hela whole cell lysate at 15 µg
Lane 3 : Hela whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 28 kDa
Exposure time: 30 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling 14-3-3 sigma/SFN with ab86380 at 1/1000 (1µg/ml). Detection: DAB.
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Detection of 14-3-3 sigma/SFN by Western blot of Immunprecipitate.
ab86380, at 1 µg/ml, staining 14-3-3 sigma/SFN in HeLa whole cell lysate immunoprecipitated using ab86380 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with an exposure time of 30 seconds.