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Neuroscience Neurology process Neural Signal Transduction

Anti-14-3-3 sigma/SFN antibody (ab86380)

Anti-14-3-3 sigma/SFN antibody (ab86380)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to 14-3-3 sigma/SFN
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-14-3-3 sigma/SFN antibody
    See all 14-3-3 sigma/SFN primary antibodies
  • Description

    Rabbit polyclonal to 14-3-3 sigma/SFN
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human 14-3-3 sigma/SFN aa 198-248.
    Database link: NP_006133.1

  • Positive control

    • HeLa whole cell lysate (ab150035)
  • General notes

     This product was previously labelled as 14-3-3 sigma

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neural Signal Transduction
    • Signal Transduction
    • Adapters
    • Cytoplasmic
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • 14-3-3

Images

  • Western blot - Anti-14-3-3 sigma/SFN antibody (ab86380)
    Western blot - Anti-14-3-3 sigma/SFN antibody (ab86380)
    All lanes : Anti-14-3-3 sigma/SFN antibody (ab86380) at 0.1 µg/ml

    Lane 1 : Hela whole cell lysate at 50 µg
    Lane 2 : Hela whole cell lysate at 15 µg
    Lane 3 : Hela whole cell lysate at 5 µg

    Developed using the ECL technique.

    Predicted band size: 28 kDa


    Exposure time: 30 seconds
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody (ab86380)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody (ab86380)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling 14-3-3 sigma/SFN with ab86380 at 1/1000 (1µg/ml). Detection: DAB.

  • Immunoprecipitation - Anti-14-3-3 sigma/SFN antibody (ab86380)
    Immunoprecipitation - Anti-14-3-3 sigma/SFN antibody (ab86380)

    Detection of 14-3-3 sigma/SFN by Western blot of Immunprecipitate.
    ab86380, at 1 µg/ml, staining 14-3-3 sigma/SFN in HeLa whole cell lysate immunoprecipitated using ab86380 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with an exposure time of 30 seconds.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-14-3-3 sigma/SFN antibody (ab86380)

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    Anti-14-3-3 sigma/SFN antibody (ab77187)

    Applications: IHC-P, WB

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    Anti-14-3-3 sigma/SFN antibody [CPTC-SFN-2] (ab268067)

    Applications: IHC-P, Protein Array, WB

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    Anti-14-3-3 sigma/SFN antibody [CPTC-SFN-2] - BSA and Azide free (ab268188)

    Applications: IHC-P, Protein Array, WB

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    Anti-14-3-3 sigma/SFN antibody (ab151504)

    Applications: ICC/IF, IHC-P, WB

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    Anti-14-3-3 sigma/SFN antibody (ab94490)

    Applications: WB

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    Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667)

    Applications: IHC-P, WB

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