Anti-Slit2 antibody [EPR2771] - BSA and Azide free (ab240085)
![Anti-Slit2 antibody [EPR2771] - BSA and Azide free (ab240085)](https://www.abcam.com/ps/products/240/ab240085/Images/ab240085-407738-AntiSlit2-antibody.png "Anti-Slit2 antibody [EPR2771] - BSA and Azide free (ab240085)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2771] to Slit2 - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Slit2 antibody [EPR2771] - BSA and Azide free
See all Slit2 primary antibodies -
Description
Rabbit monoclonal [EPR2771] to Slit2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: 293T whole cell lysate IHC: Human fetal kidney tissue
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General notes
Ab240085 is the carrier-free version of ab134166. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab240085 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2771 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Slit2 antibody [EPR2771] - BSA and Azide free (ab240085)Anti-Slit2 antibody [EPR2771] (ab134166) at 1/5000 dilution + 293T(Human embryonic kidney epithelial cell)whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 169 kDa
Observed band size: 200,55 kDa why is the actual band size different from the predicted?
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit2 antibody [EPR2771] - BSA and Azide free (ab240085)](https://www.abcam.com/ps/products/240/ab240085/Images/ab240085-326532-anti-slit2-antibody-epr2771-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit2 antibody [EPR2771] - BSA and Azide free (ab240085)Immunohistochemical analysis of paraffin embedded Human fetal kidney tissue labelled with ab134166 at 1/100 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134166).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-Slit2 antibody [EPR2771] - BSA and Azide free (ab240085)](https://www.abcam.com/ps/products/240/ab240085/Images/ab240085-3-benefits-of-recombinant-antibodies.png)
Anti-Slit2 antibody [EPR2771] - BSA and Azide free (ab240085)
