Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Slit2 antibody [SP122] - BSA and Azide free (ab242404)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [SP122] to Slit2 - BSA and Azide free
Suitable for: IHC-P, Flow Cyt
Reacts with: Mouse, Human

Product name

Anti-Slit2 antibody [SP122] - BSA and Azide free
See all Slit2 primary antibodies
Description

Rabbit monoclonal [SP122] to Slit2 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: IHC-P, Flow Cytmore details
Species reactivity

Reacts with: Mouse, Human
Predicted to work with: Pig
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human prostate adenocarcinoma tissue. Flow Cyt: SH-SY5Y cells.
General notes
FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

ab242404 is the carrier-free version of ab184856. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab242404 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.20
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from TCS by protein A/G.
Clonality

Monoclonal
Clone number

SP122
Isotype

IgG
Research areas

Flow Cytometry - Anti-Slit2 antibody [SP122] - BSA and Azide free (ab242404)

Flow Cytometry analysis of Neuro-2a (mouse neuroblastoma) cells labeling SLIT2 with purified ab184856 at 1:80 dilution (10.3 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab125938) / Black. Unlabeled control - Unlabelled cells / blue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184856).
Flow Cytometry - Anti-Slit2 antibody [SP122] - BSA and Azide free (ab242404)

Flow cytometric analysis of rabbit anti-Slit2 (SP122) antibody ab184856 diluted 1/100 in SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells (green) compare to negative control of rabbit IgG (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab184856).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit2 antibody [SP122] - BSA and Azide free (ab242404)

Staining of Slit2 in a Formalin/PFA-fixed paraffin-embedded section of human prostate adenocarcinoma tissue using ab184856 at a dilution of 1/100.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab184856).
Anti-Slit2 antibody [SP122] - BSA and Azide free (ab242404)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com