Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Tau antibody [E178] - BSA and Azide free (ab218600)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E178] to Tau - BSA and Azide free
  • Suitable for: WB, IP, IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Tau antibody [E178] - BSA and Azide free
    See all Tau primary antibodies

  • Description

    Rabbit monoclonal [E178] to Tau - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested applications

    Suitable for: WB, IP, IHC-P, IHC-Frmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Cow, Human

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: Human fetal brain lysates. IHC-P: Human brain and human cerebrum. IHC-Fr: Mouse and rat cerebrum tissue.

  • General notes

    ab218600 is the carrier-free version of ab32057. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab218600 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Frozen sections) - Anti-Tau antibody [E178] - BSA and Azide free (ab218600)
    Immunohistochemistry (Frozen sections) - Anti-Tau antibody [E178] - BSA and Azide free (ab218600)

    Immunohistochemistry (Frozen sections) analysis of rat cerebrum tissue sections labeling Tau with purified ab32057 at 1/500 (0.2 µg/ml). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32057).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [E178] - BSA and Azide free (ab218600)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [E178] - BSA and Azide free (ab218600)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cerebrum tissue sections labeling Tau with purified ab32057 at 1/4000 dilution (0.03 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32057).

  • Immunoprecipitation - Anti-Tau antibody [E178] - BSA and Azide free (ab218600)
    Immunoprecipitation - Anti-Tau antibody [E178] - BSA and Azide free (ab218600)

    ab32057 (purified) at 1/20 dilution (0.5ug) immunoprecipitating Tau in Human fetal brain lysates.
    Lane 1: Human fetal brain lysates 10ug
    Lane 2 (+): ab32057 & Human fetal brain lysates
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32057 in Human fetal brain lysates
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32057).

  • Immunohistochemistry (Frozen sections) - Anti-Tau antibody [E178] - BSA and Azide free (ab218600)
    Immunohistochemistry (Frozen sections) - Anti-Tau antibody [E178] - BSA and Azide free (ab218600)

    Immunohistochemistry (Frozen sections) analysis of mouse cerebrum tissue sections labeling Tau with purified ab32057 at 1/500 (0.2 µg/ml). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32057).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [E178] - BSA and Azide free (ab218600)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [E178] - BSA and Azide free (ab218600)

    This IHC data was generated using the same anti-Tau antibody clone, E178, in a different buffer formulation (cat# ab32057).

    Immunohistochemistical detection of Tau antibody [E178] (ab32057) on formaldehyde fixed paraffin-embedded rat tongue sectionsAntigen retrieval step: Heat mediated. Buffer Used: Citric acid pH6. Permeabilization: None. Primary antibody incubated at 1/1000 for 2 hours @ 21°C in TBS/BSA/azide. Secondary antibody: anti Rabbit IgG Conjugated to Biotin (1/200). A strong pattern of immunostaining which appears to be mostly localised to nerve fibres and their cell bodies (Islet of Langerhans cells are also positive). In submitted image of central area of tongue coloured arrowheads indicate features: red for nerves cut in cross-section (T/S), each brown dot representing a single axon green for what appears to me to be small nerve fibres wrapping around a partial muscle fibre black for a Ganglion containing seven positive nerve cell bodies. Surrounding these are collagen fibres (C), adipocytes (A) and skeletal/striated muscle fibres in L/S ( M-

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [E178] - BSA and Azide free (ab218600)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [E178] - BSA and Azide free (ab218600)

    This IHC data was generated using the same anti-Tau antibody clone, E178, in a different buffer formulation (cat# ab32057).

    Immunohistochemistical detection of Tau antibody [E178] (ab32057) on formaldehyde-fixed paraffin-embedded human salivary gland sections. Antigen retrieval step: Heat mediated.  Buffer Used: Citric acid pH6. Permeabilization: No.  Blocking step: 1% BSA for 10 mins @ 21°C. ab32057 incubated at 1/1000 for 2 hours @  21°C in TBS/BSA/azide. Secondary antibody:  anti rabbit IgG conjugated to Biotin (1/200). NB: An interesting pattern of positivity that seems to be supported by the Human Protein Atlas. Coloured arrowheads in the submitted image indicate features: red for positive serous glands, blue for positive intra-lobular collecting ducts, black for negative mucous glands (there is a serous demilune around this acinus), yellow for intralobular collecting ducts, green for nerve tracks in the interlobular areas, blue for positive interlobular collecting ducts. There appears to be a population of positive nuclei but this may b

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [E178] - BSA and Azide free (ab218600)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [E178] - BSA and Azide free (ab218600)

    Ab32057, at a dilution of 1/500, staining Tau in paraffin embedded human brain sections by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32057).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-Tau antibody [E178] - BSA and Azide free (ab218600)
    Anti-Tau antibody [E178] - BSA and Azide free (ab218600)

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