Anti-ERK1 (phospho T202) antibody (ab47310)
Key features and details
- Rabbit polyclonal to ERK1 (phospho T202)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ERK1 (phospho T202) antibody
See all ERK1 primary antibodies -
Description
Rabbit polyclonal to ERK1 (phospho T202) -
Host species
Rabbit -
Specificity
ab47310 detects endogenous levels of p44/42 MAP Kinase only when phosphorylated at threonine 202. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
synthesized phosphopeptide derived from human p44/42 MAP Kinase around the phosphorylation site of threonine 202 (F-L-TP-E-Y)
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Positive control
- Extracts from A431 cells, EGF-treated.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was purified using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ERK1 (phospho T202) antibody (ab47310)All lanes : Anti-ERK1 (phospho T202) antibody (ab47310) at 1/500 dilution
Lane 1 : EGF treated A431 cell extract (200ng/ml,30min)
Lane 2 : A431 cell extracts
Predicted band size: 43 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 (phospho T202) antibody (ab47310)ab47310 (4µg/ml) staining ERK1 (Phospho T202) in human colon.
Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 . Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
