Anti-Slit2 antibody [EPR2771] (ab134166)
![Anti-Slit2 antibody [EPR2771] (ab134166)](https://www.abcam.com/ps/products/134/ab134166/Images/ab134166-407737-AntiSlit2-antibody.png "Anti-Slit2 antibody [EPR2771] (ab134166)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2771] to Slit2
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Slit2 antibody [EPR2771]
See all Slit2 primary antibodies -
Description
Rabbit monoclonal [EPR2771] to Slit2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human Slit2 aa 1450-1550. The exact sequence is proprietary.
Database link: O94813 -
Positive control
- WB: 293T whole cell lysate IHC: Human fetal kidney tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2771 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Slit2 antibody [EPR2771] (ab134166)Anti-Slit2 antibody [EPR2771] (ab134166) at 1/5000 dilution + 293T(Human embryonic kidney epithelial cell)whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 169 kDa
Observed band size: 200,55 kDa why is the actual band size different from the predicted?Buffer and concentration: 5% NFDM /TBST
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit2 antibody [EPR2771] (ab134166)](https://www.abcam.com/ps/products/134/ab134166/Images/ab134166-148455-anti-slit2-antibody-epr2771-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit2 antibody [EPR2771] (ab134166)Immunohistochemical analysis of paraffin embedded Human fetal kidney tissue labelled with ab134166 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-Slit2 antibody [EPR2771] (ab134166)](https://www.abcam.com/ps/products/134/ab134166/Images/ab134166-4-benefits-of-recombinant-antibodies.png)
Anti-Slit2 antibody [EPR2771] (ab134166)
