Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TGIF antibody [EP637Y] - BSA and Azide free (ab236005)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP637Y] to TGIF - BSA and Azide free
  • Suitable for: IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-TGIF antibody [EP637Y] - BSA and Azide free
    See all TGIF primary antibodies

  • Description

    Rabbit monoclonal [EP637Y] to TGIF - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    ab52955 detects both phosphorylated and unphosphorylated TGIF.

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: K562 cell lysate.

  • General notes

    ab236005 is the carrier-free version of ab52955 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab236005 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Immunoprecipitation - Anti-TGIF antibody [EP637Y] - BSA and Azide free (ab236005)
    Immunoprecipitation - Anti-TGIF antibody [EP637Y] - BSA and Azide free (ab236005)

    ab52955 (purified) at 1:100 dilution (2µg) immunoprecipitating TGIF in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab52955 & HeLa whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52955 in HeLa whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52955).

  • Western blot - Anti-TGIF antibody [EP637Y] - BSA and Azide free (ab236005)
    Western blot - Anti-TGIF antibody [EP637Y] - BSA and Azide free (ab236005)
    Anti-TGIF antibody [EP637Y] (ab52955) at 1/1000 dilution + K562 cell lysate at 10 µg

    Secondary
    goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 45 kDa
    Observed band size: 30 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52955).

  • Anti-TGIF antibody [EP637Y] - BSA and Azide free (ab236005)
    Anti-TGIF antibody [EP637Y] - BSA and Azide free (ab236005)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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