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Neuroscience Neurology process Neural Signal Transduction

Anti-TGIF antibody [EP637Y] (ab52955)

Price and availability

291 484 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-TGIF antibody [EP637Y] (ab52955)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP637Y] to TGIF
  • Suitable for: WB, IP
  • Reacts with: Human

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Overview

  • Product name

    Anti-TGIF antibody [EP637Y]
    See all TGIF primary antibodies
  • Description

    Rabbit monoclonal [EP637Y] to TGIF
  • Host species

    Rabbit
  • Specificity

    ab52955 detects both phosphorylated and unphosphorylated TGIF.
  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human TGIF aa 350-450 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: HeLa and K562 cell lysate. IP: HeLa whole cell lysate (ab150035)
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP637Y
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neural Signal Transduction
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Co-activators/co-repressors
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Retinoic & Retinoid
    • Stem Cells
    • Embryonic Stem Cells
    • Intracellular
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • SMADs
    • Stem Cells
    • Neural Stem Cells
    • Intracellular
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Developmental Biology
    • Embryogenesis
    • Embryonic stem cells
    • Surface molecules

Images

  • Immunoprecipitation - Anti-TGIF antibody [EP637Y] (ab52955)
    Immunoprecipitation - Anti-TGIF antibody [EP637Y] (ab52955)

    ab52955 (purified) at 1:100 dilution (2µg) immunoprecipitating TGIF in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab52955 & HeLa whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52955 in HeLa whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-TGIF antibody [EP637Y] (ab52955)
    Western blot - Anti-TGIF antibody [EP637Y] (ab52955)
    Anti-TGIF antibody [EP637Y] (ab52955) at 0.4 µg/ml (purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 45 kDa



    Blocking and diluting buffer: 5% NFDM/TBST. 

  • Western blot - Anti-TGIF antibody [EP637Y] (ab52955)
    Western blot - Anti-TGIF antibody [EP637Y] (ab52955)
    Anti-TGIF antibody [EP637Y] (ab52955) at 1/1000 dilution (Unpurifed) + K562 cell lysate at 10 µg

    Secondary
    goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 45 kDa
    Observed band size: 30 kDa
    why is the actual band size different from the predicted?

  • Anti-TGIF antibody [EP637Y] (ab52955)
    Anti-TGIF antibody [EP637Y] (ab52955)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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