Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Dibromotyrosine antibody [9F12] (ab240911)

Key features and details

Mouse monoclonal [9F12] to Dibromotyrosine
Suitable for: WB
Reacts with: Human
Isotype: IgG1

Product name

Anti-Dibromotyrosine antibody [9F12]
See all Dibromotyrosine primary antibodies
Description

Mouse monoclonal [9F12] to Dibromotyrosine
Host species

Mouse
Specificity

Does not cross-react with Nitrotyrosine.
Tested applications

Suitable for: WBmore details
Species reactivity

Reacts with: Human
Immunogen

Chemical/ Small Molecule corresponding to Dibromotyrosine conjugated to keyhole limpet haemocyanin.
Positive control
- WB: Dibromotyrosine-BSA; HeLa cells treated with hydrogen peroxide.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein G purified
Purification notes

Purified from TCS.
Clonality

Monoclonal
Clone number

9F12
Isotype

IgG1

Western blot - Anti-Dibromotyrosine antibody [9F12] (ab240911)

All lanes : 2 hours at RT at 1/1000 dilution

Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : Hydrogen peroxidase treated HeLa cell lysate

Lysates/proteins at 12 µg per lane.

Secondary
All lanes : Goat Anti-Mouse IgG-HRP for 60 minutes at RT at 1/2000 dilution

Developed using the ECL technique.

Exposure time: 5 minutes

Block: 5% Skimmed milk in TBST.
Western blot - Anti-Dibromotyrosine antibody [9F12] (ab240911)

All lanes : Anti-Dibromotyrosine antibody [9F12] (ab240911) at 1/1000 dilution (2 hours at RT)

Lane 1 : BSA
Lane 2 : Nitrosylated-BSA
Lane 3 : Dibromotyrosine-BSA

Lysates/proteins at 1 µg per lane.

Secondary
Lanes 1-2 : Goat Anti-Mouse IgG-HRP for 60 minutes at RT. at 1/2000 dilution
Lane 3 : Goat Anti-Mouse IgG-HRP for 60 minutes at RT at 1/2000 dilution

Developed using the ECL technique.

Exposure time: 5 minutes

Block: 5% Skimmed milk in TBST.

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