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Anti-Dibromotyrosine antibody [9F12] (ab240911)

Key features and details

  • Mouse monoclonal [9F12] to Dibromotyrosine
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Dibromotyrosine antibody [9F12]
    See all Dibromotyrosine primary antibodies

  • Description

    Mouse monoclonal [9F12] to Dibromotyrosine

  • Host species

    Mouse

  • Specificity

    Does not cross-react with Nitrotyrosine.

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Chemical/ Small Molecule corresponding to Dibromotyrosine conjugated to keyhole limpet haemocyanin.

  • Positive control

    • WB: Dibromotyrosine-BSA; HeLa cells treated with hydrogen peroxide.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    Purified from TCS.

  • Clonality

    Monoclonal

  • Clone number

    9F12

  • Isotype

    IgG1

Images

  • Western blot - Anti-Dibromotyrosine antibody [9F12] (ab240911)
    Western blot - Anti-Dibromotyrosine antibody [9F12] (ab240911)
    All lanes : 2 hours at RT at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 2 : Hydrogen peroxidase treated HeLa cell lysate

    Lysates/proteins at 12 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG-HRP for 60 minutes at RT at 1/2000 dilution

    Developed using the ECL technique.

    Exposure time: 5 minutes


    Block: 5% Skimmed milk in TBST.

  • Western blot - Anti-Dibromotyrosine antibody [9F12] (ab240911)
    Western blot - Anti-Dibromotyrosine antibody [9F12] (ab240911)
    All lanes : Anti-Dibromotyrosine antibody [9F12] (ab240911) at 1/1000 dilution (2 hours at RT)

    Lane 1 : BSA
    Lane 2 : Nitrosylated-BSA
    Lane 3 : Dibromotyrosine-BSA

    Lysates/proteins at 1 µg per lane.

    Secondary
    Lanes 1-2 : Goat Anti-Mouse IgG-HRP for 60 minutes at RT. at 1/2000 dilution
    Lane 3 : Goat Anti-Mouse IgG-HRP for 60 minutes at RT at 1/2000 dilution

    Developed using the ECL technique.

    Exposure time: 5 minutes


    Block: 5% Skimmed milk in TBST.

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