HRP Anti-IKK beta antibody [Y466] (ab194713)
![HRP Anti-IKK beta antibody [Y466] (ab194713)](https://www.abcam.com/ps/products/194/ab194713/Images/ab194713-313067-ab194713KOWBJRIZ.jpg "HRP Anti-IKK beta antibody [Y466] (ab194713)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [Y466] to IKK beta
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Conjugation: HRP
Overview
-
Product name
HRP Anti-IKK beta antibody [Y466]
See all IKK beta primary antibodies -
Description
HRP Rabbit monoclonal [Y466] to IKK beta -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Daudi cell lysate and human cervical carcinoma. IHC-P: FFPE human breast adenocarcinoma.
-
General notes
Alternative versions available:
Anti-IKK beta antibody [Y466] (ab32135) - Knockout validatedOur RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Y466 -
Isotype
IgG -
Research areas
Images
-
Western blot - HRP Anti-IKK beta antibody [Y466] (ab194713)All lanes : HRP Anti-IKK beta antibody [Y466] (ab194713) at 1/5000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : IKBKB (IKK beta) knockout HAP1 whole cell lysate
Lysates/proteins at 1/20 dilution per lane.
Predicted band size: 87 kDa
Observed band size: 87 kDa
Exposure time: 20 minutesab194713 was shown to recognize IKK beta in wild-type HAP1 cells as signal was lost at the expected MW in IKBKB (IKK beta) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and IKBKB (IKK beta) knockout samples were subjected to SDS-PAGE. Ab194713 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
-
![Western blot - HRP Anti-IKK beta antibody [Y466] (ab194713)](https://www.abcam.com/ps/products/194/ab194713/Images/ab194713-233967-WBab1947131.jpg)
Western blot - HRP Anti-IKK beta antibody [Y466] (ab194713)HRP Anti-IKK beta antibody [Y466] (ab194713) at 1/5000 dilution + Daudi (Human Burkitt's lymphoma cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 87 kDa
Observed band size: 87 kDa
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% milk before being incubated with ab194713 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
-
![HRP Anti-IKK beta antibody [Y466] (ab194713)](https://www.abcam.com/ps/products/194/ab194713/Images/ab194713-4-benefits-of-recombinant-antibodies.png)
HRP Anti-IKK beta antibody [Y466] (ab194713)
