Anti-IKK beta antibody (ab264239)
Key features and details
- Rabbit polyclonal to IKK beta
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-IKK beta antibody
See all IKK beta primary antibodies -
Description
Rabbit polyclonal to IKK beta -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
-
Immunogen
Synthetic peptide within Human IKK beta aa 706-756. The exact sequence is proprietary. NP_001547.1
Database link: O14920 -
Positive control
- WB: HeLa, Jurkat, MCF7 and HEK-293T whole cell lysates. IP:Jurkat whole cell lysate.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-IKK beta antibody (ab264239)All lanes : Anti-IKK beta antibody (ab264239) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 87 kDa
Exposure time: 3 minutes
-
Immunoprecipitation - Anti-IKK beta antibody (ab264239)IKK beta was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264239 at 3 μg per reaction. Western blot was performed on the immunoprecipitates using ab264239 at 1 μg/ml.
Lane 1: ab264239 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.
Exposure time: 30 seconds.
