Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12621] to Abhd5/CGI-58
  • Suitable for: WB, Flow Cyt, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Abhd5/CGI-58 antibody [EPR12621]
    See all Abhd5/CGI-58 primary antibodies

  • Description

    Rabbit monoclonal [EPR12621] to Abhd5/CGI-58

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HepG2 cells and cell lysates; U87-MG and Human fetal liver cell lysates; Human lipomyoma tissue.

  • General notes

     

     

    Previously labelled as Abhd5. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)
    Western blot - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)
    All lanes : Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : U87-MG cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 39 kDa

  • Western blot - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)
    Western blot - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)
    Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739) at 1/1000 dilution + Human fetal liver lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 39 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)

    Immunohistochemical analysis of paraffin-embedded Human lipomyoma tissue labeling Abhd5/CGI-58 with ab183739 at 1/2000 dilution. The slide is counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)
    Flow Cytometry - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)

    Flow cytometric analysis of 2% paraformaldehyde-fixed HepG2 cells labeling Abhd5/CGI-58 with ab183739 at 1/200 dilution. Goat anti-rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. Rabbit monoclonal IgG was used as the isotype control.

  • Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)
    Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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