Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4795(2)] to SLC45A3
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-SLC45A3 antibody [EPR4795(2)]
    See all SLC45A3 primary antibodies

  • Description

    Rabbit monoclonal [EPR4795(2)] to SLC45A3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human SLC45A3 aa 400-500. The exact sequence is proprietary.
    Database link: Q96JT2

  • Positive control

    • WB: Human prostate tissue lysate; IHC-P: Human benign prostatic adenocarcinoma, prostatic hyperplasia and prostate carcinoma tissues.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product was previously labelled as Prostein.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)
    Western blot - Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)
    Anti-SLC45A3 antibody [EPR4795(2)] (ab137065) at 1/1000 dilution (Purified) + Human prostate lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 59 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?



    Blocking/Diluting buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue sections labeling SLC45A3 with purified ab137065 at 1/1000 dilution (0.128 µg/mL). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)

    Immunohistochemical analysis of paraffin-embedded Human prostatic adenocarcinoma tissue labelling SLC45A3 with as137065, unpurified, at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] (ab137065) This image is courtesy of an abreview submitted by Faye Doherty

    Immunohistochemical analysis of human prostate tissue sections labelling SLC45A3 with ab137065, unpurified, at a dilution of 1/2000. The sections were fixed with 10% NBF. The secondary antibody used was HRP conjugated goat anti rabbit IgG. Antigen retrieval was heat mediated using Tris/EDTA pH 9.0.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)

    Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labelling SLC45A3 with ab137065, unpurified, at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)
    Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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