Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-KMT6 / EZH2 antibody [SP129] - C-terminal (ab227648)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP129] to KMT6 / EZH2 - C-terminal
  • Suitable for: WB, IHC-P, Flow Cyt, IP
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-KMT6 / EZH2 antibody [SP129] - C-terminal
    See all KMT6 / EZH2 primary antibodies

  • Description

    Rabbit monoclonal [SP129] to KMT6 / EZH2 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa whole cell lysate (ab150035), NIH/3T3 and HEK-293 cell lysates; Mouse brain tissue lysate. IHC-P: Human prostate cancer tissue. Flow Cyt: HeLa cells. IP: HEK-293 whole cell lysate

  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-KMT6 / EZH2 antibody [SP129] - C-terminal (ab227648)
    Western blot - Anti-KMT6 / EZH2 antibody [SP129] - C-terminal (ab227648)
    All lanes : Anti-KMT6 / EZH2 antibody [SP129] - C-terminal (ab227648) at 0.316 µg/ml

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) cell lysate
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) cell lysate
    Lane 3 : Mouse brain tissue lysate
    Lane 4 : HEK-293 (Human embryonic kidney epithelial cell) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml

    Predicted band size: 85 kDa
    Observed band size: 85 kDa



    Blocking/Diluting buffer and concentration: 5% NFDM /TBST
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT6 / EZH2 antibody [SP129] - C-terminal (ab227648)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT6 / EZH2 antibody [SP129] - C-terminal (ab227648)

    Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for KMT6 / EZH2 using ab227648 at 1/100 dilution in immunohistochemical analysis.

  • Immunoprecipitation - Anti-KMT6 / EZH2 antibody [SP129] - C-terminal (ab227648)
    Immunoprecipitation - Anti-KMT6 / EZH2 antibody [SP129] - C-terminal (ab227648)

    ab227648 (purified) at 1/20 dilution (20 µg/ml) immunoprecipitating KMT6 in HEK-293 whole cell lysate.

    Lane 1 (input): HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab227648 & HEK-293 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab227648 in HEK-293 whole cell lysate

    For western blotting, ab227648 (purified at 1/500 dilution (0.632 µg/ml) and veriBlot for IP secondary antibody (HRP) (ab131366) at 1/1000 dilution were used.
    Blocking and diluting buffer: 5% NFDM /TBST.

  • Western blot - Anti-KMT6 / EZH2 antibody [SP129] - C-terminal (ab227648)
    Western blot - Anti-KMT6 / EZH2 antibody [SP129] - C-terminal (ab227648)
    Anti-KMT6 / EZH2 antibody [SP129] - C-terminal (ab227648) at 1/25 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

    Predicted band size: 85 kDa

  • Flow Cytometry - Anti-KMT6 / EZH2 antibody [SP129] - C-terminal (ab227648)
    Flow Cytometry - Anti-KMT6 / EZH2 antibody [SP129] - C-terminal (ab227648)

    Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling KMT6 / EZH2 with ab227648 at 1/100 dilution (green) compared with a negative control of rabbit IgG (blue).

  • Anti-KMT6 / EZH2 antibody [SP129] - C-terminal (ab227648)
    Anti-KMT6 / EZH2 antibody [SP129] - C-terminal (ab227648)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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