Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SLC45A3 antibody [EPR4795(2)] - BSA and Azide free (ab242056)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4795(2)] to SLC45A3 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-SLC45A3 antibody [EPR4795(2)] - BSA and Azide free
    See all SLC45A3 primary antibodies

  • Description

    Rabbit monoclonal [EPR4795(2)] to SLC45A3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human prostate tissue lysate; IHC-P: Human benign prostatic adenocarcinoma, prostatic hyperplasia and prostate carcinoma tissues.

  • General notes

    ab242056 is the carrier-free version of ab137065 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab242056 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. This product was previously labelled as Prostein

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-SLC45A3 antibody [EPR4795(2)] - BSA and Azide free (ab242056)
    Western blot - Anti-SLC45A3 antibody [EPR4795(2)] - BSA and Azide free (ab242056)
    Anti-SLC45A3 antibody [EPR4795(2)] (ab137065) at 1/1000 dilution (Purified) + Human prostate lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 59 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?



    Blocking/Diluting buffer: 5% NFDM/TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137065).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] - BSA and Azide free (ab242056)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] - BSA and Azide free (ab242056)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue sections labeling SLC45A3 with purified ab137065 at 1/1000 dilution (0.128 µg/mL). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137065).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] - BSA and Azide free (ab242056)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] - BSA and Azide free (ab242056)

    Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labelling SLC45A3 with ab137065 at 1/100 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137065).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] - BSA and Azide free (ab242056)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] - BSA and Azide free (ab242056)

    Immunohistochemical analysis of paraffin-embedded Human prostatic adenocarcinoma tissue labelling SLC45A3 with as137065 at 1/100 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137065).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] - BSA and Azide free (ab242056)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] - BSA and Azide free (ab242056)

    Immunohistochemical analysis of human prostate tissue sections labelling SLC45A3 with ab137065 at a dilution of 1/2000. The sections were fixed with 10% NBF. The secondary antibody used was HRP conjugated goat anti rabbit IgG. Antigen retrieval was heat mediated using Tris/EDTA pH 9.0.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137065).

  • Anti-SLC45A3 antibody [EPR4795(2)] - BSA and Azide free (ab242056)
    Anti-SLC45A3 antibody [EPR4795(2)] - BSA and Azide free (ab242056)

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