Anti-Abhd5/CGI-58 antibody (ab59488)
Key features and details
- Goat polyclonal to Abhd5/CGI-58
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Abhd5/CGI-58 antibody
See all Abhd5/CGI-58 primary antibodies -
Description
Goat polyclonal to Abhd5/CGI-58 -
Host species
Goat -
Specificity
This antibody is not expected to cross-react with ABHD4. -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Synthetic peptide corresponding to Human Abhd5/CGI-58 aa 181-192 (internal sequence).
Sequence:C-FPERPDLADQDR
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General notes
Previously labelled as Abhd5.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Abhd5/CGI-58 antibody (ab59488)Anti-Abhd5/CGI-58 antibody (ab59488) at 1 µg/ml + A431 cell line lysate (35µg protein in RIPA buffer)
Predicted band size: 39 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence. -
Western blot - Anti-Abhd5/CGI-58 antibody (ab59488)Anti-Abhd5/CGI-58 antibody (ab59488) at 0.2 µg/ml + NIH-3T3 cell lysate (RIPA buffer) at 35 µg
Predicted band size: 39 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
