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Anti-Dibromotyrosine antibody [6G3] (ab241051)

Key features and details

  • Mouse monoclonal [6G3] to Dibromotyrosine
  • Suitable for: IHC, WB, Dot blot
  • Isotype: IgG1

Overview

  • Product name

    Anti-Dibromotyrosine antibody [6G3]
    See all Dibromotyrosine primary antibodies

  • Description

    Mouse monoclonal [6G3] to Dibromotyrosine

  • Host species

    Mouse

  • Specificity

    Specific for 3,5-Dibromotyrosine. Does not cross-react with Nitrotyrosine.

  • Tested applications

    Suitable for: IHC, WB, Dot blotmore details

  • Immunogen

    Chemical/ Small Molecule corresponding to Dibromotyrosine conjugated to keyhole limpet haemocyanin.

  • Positive control

    • Dot Blot: 3, 5-Dibromotyrosine-BSA conjugated protein. WB: Dibromotyrosine-BSA. Hydrogen peroxidase treated HeLa cell lysate. IHC: Human liver cancer tissue.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    Purified from TCS.

  • Clonality

    Monoclonal

  • Clone number

    6G3

  • Isotype

    IgG1

Images

  • Immunohistochemistry - Anti-Dibromotyrosine antibody [6G3] (ab241051)
    Immunohistochemistry - Anti-Dibromotyrosine antibody [6G3] (ab241051)

    Immunohistochemical analysis of human liver cancer tissue labeling Dibromotyrosine with ab241051 (green). Tissue was incubated with ab241051 at 1/100 overnight at 4oC then for 30 minutes at 37oC, followed by incubation with a Goat anti-Mouse IgG (H+L):FITC secondary antibody for 45 minutes at 37oC. Counterstain used was DAPI for 3 minutes at RT. Magnification: 5X.

  • Western blot - Anti-Dibromotyrosine antibody [6G3] (ab241051)
    Western blot - Anti-Dibromotyrosine antibody [6G3] (ab241051)
    All lanes : Anti-Dibromotyrosine antibody [6G3] (ab241051) at 1/2000 dilution (2 hours at RT)

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 2 : Hydrogen peroxidase treated HeLa cell lysate

    Lysates/proteins at 12 µg per lane.

    Secondary
    All lanes : Goat anti-Mouse IgG-HRP for 60 minutes at RT at 1/2000 dilution

    Developed using the ECL technique.

    Exposure time: 5 minutes


    Block: 5% Skimmed milk in TBST.

  • Western blot - Anti-Dibromotyrosine antibody [6G3] (ab241051)
    Western blot - Anti-Dibromotyrosine antibody [6G3] (ab241051)
    All lanes : Anti-Dibromotyrosine antibody [6G3] (ab241051) at 1/1000 dilution (2 hours at RT)

    Lane 1 : BSA
    Lane 2 : Nitrosylated-BSA
    Lane 3 : Dibromotyrosine-BSA

    Lysates/proteins at 1 µg per lane.

    Secondary
    All lanes : Goat anti-Mouse IgG-HRP for 60 minutes at RT at 1/2000 dilution

    Developed using the ECL technique.

    Exposure time: 5 minutes


    Block: 5% Skimmed milk in TBST.

  • Dot Blot - Anti-Dibromotyrosine antibody [6G3] (ab241051)
    Dot Blot - Anti-Dibromotyrosine antibody [6G3] (ab241051)

    Dot Blot analysis using ab241051 at 1/1000 dilution for 2 hours at RT.

    Secondary Antibody: Goat anti-Mouse HRP:IgG at 1/4000 dilution for 1 hour at RT.

    The quantities of protein spotted on each panel are as shown.

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