Anti-Dibromotyrosine antibody [6G3] (ab241051)
![Anti-Dibromotyrosine antibody [6G3] (ab241051)](https://www.abcam.com/ps/products/241/ab241051/Images/ab241051-451403-anti-dibromotyrosine-antibody-6g3-immunohistochemistry-liver-human.jpg "Anti-Dibromotyrosine antibody [6G3] (ab241051)")
Key features and details
- Mouse monoclonal [6G3] to Dibromotyrosine
- Suitable for: IHC, WB, Dot blot
- Isotype: IgG1
Overview
-
Product name
Anti-Dibromotyrosine antibody [6G3]
See all Dibromotyrosine primary antibodies -
Description
Mouse monoclonal [6G3] to Dibromotyrosine -
Host species
Mouse -
Specificity
Specific for 3,5-Dibromotyrosine. Does not cross-react with Nitrotyrosine. -
Tested applications
Suitable for: IHC, WB, Dot blotmore details -
Immunogen
Chemical/ Small Molecule corresponding to Dibromotyrosine conjugated to keyhole limpet haemocyanin.
-
Positive control
- Dot Blot: 3, 5-Dibromotyrosine-BSA conjugated protein. WB: Dibromotyrosine-BSA. Hydrogen peroxidase treated HeLa cell lysate. IHC: Human liver cancer tissue.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
6G3 -
Isotype
IgG1
Images
-
Immunohistochemistry - Anti-Dibromotyrosine antibody [6G3] (ab241051)
Immunohistochemical analysis of human liver cancer tissue labeling Dibromotyrosine with ab241051 (green). Tissue was incubated with ab241051 at 1/100 overnight at 4oC then for 30 minutes at 37oC, followed by incubation with a Goat anti-Mouse IgG (H+L):FITC secondary antibody for 45 minutes at 37oC. Counterstain used was DAPI for 3 minutes at RT. Magnification: 5X.
-
![Western blot - Anti-Dibromotyrosine antibody [6G3] (ab241051)](https://www.abcam.com/ps/products/241/ab241051/Images/ab241051-319822-24105144.jpg)
Western blot - Anti-Dibromotyrosine antibody [6G3] (ab241051)All lanes : Anti-Dibromotyrosine antibody [6G3] (ab241051) at 1/2000 dilution (2 hours at RT)
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : Hydrogen peroxidase treated HeLa cell lysate
Lysates/proteins at 12 µg per lane.
Secondary
All lanes : Goat anti-Mouse IgG-HRP for 60 minutes at RT at 1/2000 dilution
Developed using the ECL technique.
Exposure time: 5 minutesBlock: 5% Skimmed milk in TBST.
-
![Western blot - Anti-Dibromotyrosine antibody [6G3] (ab241051)](https://www.abcam.com/ps/products/241/ab241051/Images/ab241051-319821-24105133.jpg)
Western blot - Anti-Dibromotyrosine antibody [6G3] (ab241051)All lanes : Anti-Dibromotyrosine antibody [6G3] (ab241051) at 1/1000 dilution (2 hours at RT)
Lane 1 : BSA
Lane 2 : Nitrosylated-BSA
Lane 3 : Dibromotyrosine-BSA
Lysates/proteins at 1 µg per lane.
Secondary
All lanes : Goat anti-Mouse IgG-HRP for 60 minutes at RT at 1/2000 dilution
Developed using the ECL technique.
Exposure time: 5 minutesBlock: 5% Skimmed milk in TBST.
-
![Dot Blot - Anti-Dibromotyrosine antibody [6G3] (ab241051)](https://www.abcam.com/ps/products/241/ab241051/Images/ab241051-319820-24105122.jpg)
Dot Blot - Anti-Dibromotyrosine antibody [6G3] (ab241051)Dot Blot analysis using ab241051 at 1/1000 dilution for 2 hours at RT.
Secondary Antibody: Goat anti-Mouse HRP:IgG at 1/4000 dilution for 1 hour at RT.
The quantities of protein spotted on each panel are as shown.
